Dose dependant anti-inflammatory effects of Dehydrocostus Lactone
- VernacularTitle:Дегидрокостус лактонын тун хамааралт үрэвслийн эсрэг үйлдэл
- Author:
Fuquan Zhao
1
,
2
;
Shiirevnyamba A
1
;
Bilegtsaikhan Ts
1
;
Tovuudorj A
1
;
Seesregdorj S
1
Author Information
1. Mongolian National University of Medical Sciences
2. Hospital of Inner Mongolian University for the Nationalities
- Publication Type:Journal Article
- Keywords:
Microglia;
Dehydrocostus Lactone;
lipopolysaccharide;
IL-1β;
IL-6;
TNF-α
- From:Mongolian Medical Sciences
2019;188(2):42-46
- CountryMongolia
- Language:Mongolian
-
Abstract:
Introduction:After central nervous system injury, microglia cells are activated to initiate inflammatory responses and
release cytokines that beneficially or detrimentally affect surrounding cells. Lipopolysaccharide stimulates
microglia cells and produce pro-inflammatory cytokines such as interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF)-α. A dehydrocostus lactone (DDL) which is contained in medicinal plant, Saussurea lappa, is considered to have various health benefits in neurodegenerative diseases of central nervous system.
In this study, we aimed to investigate the anti-inflammatory effects of Dehydrocostus Lactone following
lipopolysaccharide stimulation of microglial cells in vitro.
Materials and Method:The anti-inflammatory effects of dehydrocostus lactone were studied using lipopolysaccharide (LPS)
stimulated murine microglia (BV2). BV2 were cultered in DMEM then three different doses (4µM, 8µM and
12µM) of DDL were added in the medium for 30 minutes respectively. Then BV2 were treated with 1 ng/
ml LPS for 24 hours to stimulate. The level of IL-1β, IL-6 and TNF-α were measured in 100µl of culturemedium supernatant by ELISA. Three different doses of DDL anti-inflammation groups (BV2+DDL+LPS), LPS-activated group (BV2+LPS) and control group (only BV2) were analysed.
Results:LPS-treated BV2 cells had increased IL-1β, IL-6 and TNF-α compared with those without LPS treatment.
Pretreatment with dehydrocostus lactone prior to LPS treatment significantly decreased levels of IL-1β
and TNF-α in a dose-dependent manner compared with LPS-treated BV2 cells and 4µM was the most
effective anti-inflammatory dose of dehydrocostus lactone. As for IL-6, 12µM dehydrocostus lactone was
the most effective anti-inflammatory dose, although all doses significantly decreased the level of IL-6, in a
dose-dependent manner.
Conclusion:These results show that DDL decrease inflammation related IL-1β, IL-6 and TNF-α in a dose-dependent
manner in microglia cells.
- Full text:2019-188(2);42-46.pdf
