The study of the intestinal microbiota among Mongolian Adults, it related some factors
- VernacularTitle:Насанд хүрсэн Монгол хүний бүдүүн гэдэсний хэвийн нянгийн бүрдэл, түүнд нөлөөлөх зарим хүчин зүйлс
- Author:
Saruuljavkhlan B
1
,
2
;
Battogtokh Ch
3
;
Enkh-Amar A
2
;
Otgonjargal B
3
;
Otgonbileg J
3
;
Anuujin G
3
;
Gerelmaa E
3
;
Bira N
2
Author Information
1. Mongolian Institute of Medical Sciences
2. School of Biomedicine, Mongolian National University of Medical Sciences
3. School of Medicine, Mongolian National University of Medical Sciences
- Publication Type:Journal Article
- Keywords:
Bifidobacterium;
Colony forming unit;
Gut microbiota;
Lactobacillus
- From:Mongolian Medical Sciences
2019;188(2):3-11
- CountryMongolia
- Language:Mongolian
-
Abstract:
Background:The intestinal microbiota of Mongolians and its composition is of great interest of researchers, a few studies
have did in this fields. Maybe Mongolian encompass a uniquely wide range of environmental conditions, ethno
geographical cohorts and traditional nomadic lifestyles.
Goal:We aimed to determine the amount of gut microbiota, including Lactobacillus and Bifidobacterium in the fecal
samples of relative healthy Mongolian adults residing in various regions of Mongolia by conventional culture
method and PCR.
Material and Methods:The study was performed population based cross sectional study in healthy volunteers. In this study, 256
relative healthy Mongolian adults with no history of gastrointestinal associated diseases were enrolled
between July 2018 and April 2019. Each participants was asked to complete a questionnaire containing 164
questions about demographics, physical activity, dietary habits. Fecal samples were collected for Lactobacillus
and Bifidobacterium analysis using culture method and determination of genus of Bifidobacterium sрp and
Lactobacillus spp by PCR.
ResultsParticipants had a mean age of 38.9±12.8 years. The mean values of Lactobacillus
by culture method were 5.9±1.28 and 6.24±0.94 log10 CFU/ml (4.67х106
, 4.66х106 CFU/ml), respectively. The
abundance of Lactobacillus had a positive correlation with grams for fiber and amount of bifidobacterium ((r=
0.495, р<0.001, r=0.288, p<0.05), respectively). Significant difference were observed between groups of milk
frequency per day for amounts of lactobacillus. In adult intestinal tracts, B.Bifidum was the most common taxon
31 (29%) followed by B. angulatum 14 (13.1%), B. adolescentis 10 (9.3%), B. catenulatum group 10 (9.3%), B.
longum 9 (8.4%). B. lactis, B. breve, B. dentium and B. gallicum were subdominant species.
Conclusion: :The mean amount of Bifidobacterium and Lactobacillus of all participants were 6.24±0.94 and 5.9±1.28 log10
CFU/ml (4.66*106
, 4.67*106
CFU/ml) respectively. The Lactobacillus abundance of healthy adults was higher in
region of Khangai, East and West of Mongolian than other regions. The composition of lactobacillus altered with
ageing. Significant correlations were found between fiber, fats, potato and amount of Lactobacillus.
Keywords: Bifidobacterium, Colony forming unit, Gut microbiota, Lactobacillus
- Full text:2019-188(2)-3-111.pdf
