Technological study for obtaining an appropriate extract from Stellera Chamaejasme L. and Oxytropis Pseudoglandulosa
- VernacularTitle:Одой далан түрүү (Stellera Chamaejasme L.), хуурмаг булчирхайт ортууз (Oxytropis Pseudoglandulosa)-аастохиромжтой ханд гарган авах технологийн судалгааны дүнгээс
- Author:
Maitsetseg N
1
;
Ariuntsetseg A
1
;
Altantuya Ts
1
;
Ulambayar L
1
;
Altansukh Ts
2
;
Tserendolgor B
1
Author Information
1. Mongolian University of Pharmaceutical Sciences
2. Mongolian National University of Medical Sciences
- Publication Type:Journal Article
- Keywords:
Extract;
total flavonoid;
thin layer chromatography;
spectrophotometer
- From:
Mongolian Pharmacy and Pharmacology
2023;22(1):22-27
- CountryMongolia
- Language:Mongolian
-
Abstract:
Background:Infections in respiratory systems have spread throughout the world without any restrictions including living places, public issues, and lifestyle. Three main causes of illnesses for the population of cities and rural areas were gastrointestinal diseases, respiratory diseases, and cardiovascular diseases. After investigated some medicinal herbs including Stelleria Chamaejasme L. and Oxytropis Pseudoglandulosa, it has been reported that they had antiinflammatory, analgesic, and wound healing effects. Lozenge formulation has some advantages for treatment application, such as easily absorbed, good bioavailability and ability of diminishing stomach irritation. In this study, we aimed to obtain a suitable extract from Stelleria Chamaejasme L. and Oxytropis Pseudoglandulosa for further lozenge formulation.
Purpose:To obtain a suitable extract from Stelleria Chamaejasme L. and Oxytropis Pseudoglandulosa, and to conduct qualitative and quantitative studies for some biologically active substances
Materials and methods:In this study, an aerial part of Stelleria Chamaejasme L. and Oxytropis Pseudoglandulosa were used, and the study was conducted in MUPS. For obtaining a suitable extract, the raw materials were extracted by remaceration, repercolation and circulation methods in 20% and 70% of ethanol and distilled water. The flavonoids and polyphenolic compounds in the extracts were determined by thin layer chromatography. Quantitative analysis for total flavonoids was performed by spectrophotometer.
Results:According to the result, a yellow spot-on chromatogram was detected in extracted raw materials (Stelleria Chamaejasme L. and Oxytropis Pseudoglandulosa), indicating that flavonoid contained in the extracted solution.
The result was compared to standards of rutin (Rf=0.2) and quercetin (Rf= 0.94). Also, a black, blue spot-on chromatogram was detected in extracted raw materials (Stelleria Chamaejasme L. and Oxytropis Pseudoglandulosa), indicating that polyphenols contained in the extracted solution. The spots were compared to gallic acid as a standard substance. In the quantitative assay of total flavonoids in raw materials, black-green precipitation was revealed after procedure. From this result, remaceration and circulation techniques were suitable to extract the raw materials. Flavonoid content was 3.35±0.04% after using remaceration technique, which indicated that it was more suitable to extract the raw materials.
Conclusions:These results showed that the appropriate extracting solution for Stelleria Chamaejasme L. and Oxytropis Pseudoglandulosa was 70% of ethanol. In this case, 3.35±0.04% of flavonoid was extracted by remaceration technique.
- Full text:MPPJ-2023-22(1)-22-27.pdf
