The result on anatomical study of Inula Helenium
- VernacularTitle:Өндөр зоосонцэцэг (Inula Helenium L.) - ийн ургал эрхтний анатомийн судалгааны үр дүнгээс
- Author:
Densmaa B
1
;
Gantogtokh G
1
;
Khatanzorig N
1
;
Kristina Amalia
1
Author Information
1. Mongolian University of Pharmaceutical Sciences
- Publication Type:Journal Article
- Keywords:
Inula Helenium;
leaves;
stem;
flower;
anatomy
- From:
Mongolian Pharmacy and Pharmacology
2022;20(1):72-77
- CountryMongolia
- Language:Mongolian
-
Abstract:
Introduction:In recent years, some species of plants that are used in traditional medicine and have high practical value have been successfully introduced in our country. It is necessary to carry out phytochemical studies of local medicinal and useful plants to produce food and biologically active food supplements. The tall coin flower (Inula Helenium L.) has been successfully cultivated in Mongolia, and the lower layer of the plant such as roots and stems are used. However, most of the times the top layer of the plant is not used and thrown away, that is why the plant must be fully utilized. Therefore, the aim of this study is to analyze the top layer of the plant and present the results of anatomical studies.
Methods:Remove the top layer of the plant and soak it in glycerin for 24 hours. When making an incision, select a green, undamaged part of the plant and place it in the VCM-202III freezer microtome. Chloral hydrate liquid should be used for micro-preparation. In addition to implementing chloral hydrate solution, use the solutions of various concentrations of sodium alkali (NaOH) (5- 15%). Place the slice in alcian blue stain (using the cell wall staining method) for 3 minutes and wash it with distilled water. Then stain it in a drop of safranin staining (0.5–1.0% aqueous solution) for 1 minute. Rinse it twice with distilled water, add a mixture of glycerin and distilled water, cover it with a glass slide and prepare a temporary slide. Examine the prepared temporary slide with a NOVEL light microscope. Cell images of the plant’s anatomical structures are captured on a computer screen with the help of a digital camera.
Conclusion:The anatomy of the leaves, stems and flowers of the tall coin flower (Inula Helenium L.), a plant cultivated in Mongolia, has been analyzed.
- Full text:MPPJ-2022-20(1)-72-77.pdf
