Effect of “shilajit+golden rosa” or vitos preparation on immune response cells (CD4, CD8, IGG, CD64) by azathioprine induced immunosuppression in mice
- VernacularTitle:Хулганад азатиопринээр үүсгэсэн дархлаа дутагдлын үед дархлааны эс (CD4, CD8, IGG, CD64)-ийн хариу урвалд “барагшун+алтан гагнуур” витос бэлдмэлийн үзүүлэх нөлөө
- Author:
Batchimeg B
1
;
Davaasambuu T
1
;
Badamtsetseg S
1
;
Bayanmunkh A
1
;
Odongerel A
2
;
Tsetsegmaa S
1
;
Choijamts G
1
;
Lkhagva L
1
;
Khurelbaatar L
1
,
3
Author Information
1. Drug Research Institute
2. Monos Food JSC
3. Monos Group
- Publication Type:Journal Article
- Keywords:
Cytokine CD4;
Cytokine CD8;
Immunostimulatory;
Azathioprine;
Immunoglobulin G.
- From:
Mongolian Pharmacy and Pharmacology
2022;20(1):39-44
- CountryMongolia
- Language:Mongolian
-
Abstract:
Introduction:During many decides, compounds derived from natural raw materials have demonstrated their effectiveness as therapeutic agents in different areas, such as metabolic disorder, immune system diseases and its regulations. Natural based products, like herbal medicines and minerals are implicated in the regulation of immune function. They control the immune system in a pleiotropic manner and participate in various processes of the adaptive/innate immunity. Therefore, natural raw material has great potential for targeted immune modulators, in the treatment of certain types of immunologic and inflammatory diseases, like rheumatoid arthritis, plaque psoriasis, ankylosing spondylitis and immune deficiency. The purpose of this survey was to study influence of “Shilajit +Golden Rosa” combined shot preparation named by Vitos on immune system in the experimental and preclinical circumstances.
Goal:The purpose of this survey was to study influence of “Shilajit +Golden Rosa” Vitos shot preparation
on immune system in the experimental and preclinical circumstances.
Material and Methods:The immune deficiency was to created by Azathioprine through 5 days in the Balb/c mice after that control group, preparation of “Shilajit +Golden Rosa” Vitos shot were administrated appropriate doses by oral during 10 days. Then we collected blood and quantified amount of CD4+, CD8+, IgG and CD64 (Mouse Elisa Kit Assay: Catalog.No:WAM-568, Elisa Reader, 450 нм, Melsin Medical Co.LTD, www. melsin.com) on the 5th, 10th days.
Results:All statistical analyses were conducted with SPSS version 20.0 software (IBM, Armonk, NY). Oneway ANOVA was used to assess statistical significance between “Shilajit +Golden Rosa” Vitos shot group and days of observation. Mean values of CD4+, CD8+, CD4/CD8 ratio, IgG, CD64 levels determined in the control and sample group. CD4+, CD8+, CD4/CD8 ratio, IgG and CD64 levels were significantly increased in the “Shilajit +Golden Rosa” Vitos shot group compared with control group by 20.8-67.8 per cent (p<0.05, p<0.01).
Conclusion:It’s concluded that, “Shilajit +Golden Rosa” Vitos shot preparation shows immune-stimulator activity not only in the level of cellular (T cells:CD4+, CD8+) but also humoral immunity (B cells: IgG, CD64) in the previously using Azathioprine (75mg/kg) to provoke pathological model of immunosuppression
- Full text:MPPJ-2022-20(1)-39-44.pdf
