Study of Screening Differential Genes of Malignant Melanoma Using Gene Chip Expression Profiles
- VernacularTitle:用基因芯片表达谱筛选恶性黑色素瘤差异基因的研究
- Author:
Lin-xi ZHANG
;
Cheng-gang YI
;
Xu-dong ZHANG
;
Li YANG
;
Jiqing YANG
;
Shuzhong GUO
- Publication Type:Journal Article
- Keywords:
gene chip technique, malignant melanoma, nevus, differential gene
- From:
Chinese Journal of Rehabilitation Theory and Practice
2006;12(8):694-695
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo identify differential genes of malignant melanoma using gene chip expression profiles.MethodsAgilent Human 1A OligoDNA was employed to find out difference in gene expression between malignant melanoma and nevus. The total RNA was isolated from two type tissues, labled the fluorescent to the probe, hybridized, washed and analyzed.ResultsAmong the 21073 target genes, 1596 genes were differentially expressed in malignant melanoma, including 733 genes up-regulated, and 863 down-regulated.ConclusionThe gene chip technique can screen genes that may be specifically expressed in malignant melanoma.
