Separateness and Directional Adipocyte Differentiation of Murine Bone Marrow Mesenchymal Stem Cells
- VernacularTitle:小鼠骨髓间充质干细胞的分离及其诱导定向脂肪细胞分化
- Author:
Chang-liang JIAO
;
Yang-de ZHANG
;
Qi-yang XIE
;
Yifeng PAN
- Publication Type:Journal Article
- Keywords:
murine, mesenchymal stem cells, adipocyte, tissue engineering
- From:
Chinese Journal of Rehabilitation Theory and Practice
2006;12(8):682-684
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo establish separate and appraisal methods of murine bone marrow mesenchymal stem cells (MSCs) and optimize the suitable conditions inducting MSCs directional differentiating into adipocytes in vitro.MethodsThe differential adherence to plastic was employed to separate MSCs. CFU-f and successive CFU-f cultures were employed to characterize the potent of proliferation and self-renewal of MSCs. The different adipogenic medium was used as induction for the differentiation of MSCs into adipocytes. The differentiated cells were identified by oil red O immunohistochemistry stain.ResultsThe purified MSCs showed the morphology of fibroblasts. It was found that the number of CFU-f formation depended on the planted number of MSCs. It showed a good relationship. Small type colony of CFU-f had little potent to re-clone, but almost 90% big type colony of CFU-f had the potent to regenerate CFU-f. The MSCs could directionally differentiated into adipocytes induced by different adipogenic medium. But more than 96% MSCs differentiated into mature adipocytes when induced by combined with dexamethasone (DM), 1-methy-3-isobutylxanthine (IBMX), insulin (IS) and indomethacin (ID).ConclusionThe purified MSCs can be harvested by method of differential adherence to plastic, and these MSCs have the potent of proliferation and self-renewal. Moreover, more than 96% MSCs can differentiate into mature adipocytes when induced by combined with DM, IBMX, IS and ID.
