Bladder Regeneration by Small Intestinal Submucosa with Release of Exogenous Growth Factors
- VernacularTitle:小肠粘膜下层在膀胱组织修复中生长因子释放的研究
- Author:
Mao-hu LIN
;
Hua-liang YU
;
Rui MIAO
;
Wanzhang QIN
;
Ning JIA
;
Juan LI
- Publication Type:Journal Article
- Keywords:
small intestinal submucosa (SIS), bladder, regeneration, vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF), rats
- From:
Chinese Journal of Rehabilitation Theory and Practice
2006;12(7):578-580
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the release of exogenous growth factors from small intestinal submucosa (SIS) in bladder regeneration. MethodsThe release of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) from SIS in vitro were evaluated by ELISA and MTT method. The defected bladder walls of rats in experimental group were repaired with porcine small intestinal submuscosa. Partial bladder mucosa and smooth muscle of the rats in control groups were destroyed. At regular intervals, the VEGF and bFGF expression were observed by histological and immunohistochemical methods. ResultsThe concentration of bFGF and VEGF released in vitro from SIS in PBS solution were (121.8±2.683) ng/L and (93.8±3.033) ng/L respectively, and showed proliferation of vascular endothelial cell. In the SIS framework, the capillary and smooth muscle were observed followed histological evaluation. The weak expression of VEGF and bFGF in both experimental and control groups were found in the first week. Since the second week the VEGF and bFGF expression in experimental group began to increase with a peak in the 6th week, and began to decrease after 8 weeks. In the control group, the weak VEGF and bFGF expression were shown during the observation. ConclusionSIS functions as a carrier for exogenous growth factors release in rat bladder regeneration.
