Development and verification of double antibody sandwich ELISA for quantitative detection of TrypLE
10.13200/j.cnki.cjb.003831
- VernacularTitle:TrypLE双抗体夹心定量ELISA检测方法的建立及验证
- Author:
BI Hua
- Publication Type:Journal Article
- Keywords:
Double antibody sandwich ELISA;
TrypLE;
Quantitative detection
- From:
Chinese Journal of Biologicals
2023;36(2):187-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop and verify a double antibody sandwich ELISA method for quantitative detection of TrypLE.Methods The optimal concentration of capture antibody and detection antibody were determined by orthogonal experiments to develop TrypLE double antibody sandwich ELISA quantitative detection method,which was verified for linear range,specificity,limit of detection(LOD),limit of quantitation(LOQ),accuracy and reproducibility.A variety of biological products were detected by the developed method to verify the applicability.Results The TrypLE double antibody sandwich ELISA quantitative detection method was established by using 3 μg/mL capture antibody and 15 000 times dilution of detection antibody,with a linear range of 0.41 ～ 40.00 ng/mL,a LOD of 0.258 ng/mL,a LOQ of 0.5 ng/mL.The measurement deviation was less than 5% and the CV of reproducibility verification was less than 5% when detecting standards and samples.The recovery rates of different types of samples were within 80% ～ 120%.Conclusion The established TrypLE double antibody sandwich ELISA quantitative detection method accurately,effectively and quickly detected residual amount of TrypLE in various types of biological products with good specificity,accuracy and reproducibility.
