Study on the extraction and antifungal activity of volatile oil from stems，leaves and roots of Glehnia littoralis
- VernacularTitle:北沙参茎叶和根中挥发油的提取与抗真菌活性研究
- Author:
Zhenmin YANG
1
,
2
,
3
;
Bing LI
1
,
2
;
Fuying MAO
4
;
Yuguang ZHENG
2
,
5
;
Yunsheng ZHAO
1
,
2
Author Information
1. School of Pharmacy，Hebei University of Chinese Medicine，Shijiazhuang 050200，China
2. Hebei Traditional Chinese Medicine Processing Technology Innovation Center，Hebei University of Chinese Medicine，Shijiazhuang 050091，China
3. International Joint Research Center for the Utilization and Quality Evaluation of Traditional Chinese Medicine Resources in Hebei Province，Shijiazhuang 050091，China
4. Experimental Center，Hebei University of Chinese Medicine，Shijiazhuang 050200，China
5. Hebei Chemical and Pharmaceutical College，Shijiazhuang 050026，China
- Publication Type:Journal Article
- Keywords:
Glehnia littoralis;
stems;
leaves;
roots;
volatile oil;
fungus;
antifungal activity;
chemical components
- From:
China Pharmacy
2023;34(11):1358-1362
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the extraction process of volatile oil from the stems， leaves and roots of Glehnia littoralis， analyze the chemical components of the volatile oil from the stems， leaves and roots of G. littoralis， and preliminarily evaluate its in vitro antifungal activity. METHODS Based on the steam distillation method， single factor test and orthogonal experiment were conducted to optimize the extraction method of volatile oil from the stems， leaves and roots of G. littoralis. The chemical components of the volatile oil from the stems， leaves and roots of G. littoralis were identified by using gas chromatography-mass spectrometry （GC-MS) technology and their relative contents were calculated. The antifungal activity of volatile oils from the stems， leaves and roots of G. littoralis against Fusarium solani， Fusarium incarnatum， Fusarium oxysporum， Aspergillus parasiticus and Aspergillus flavus was determined by paper diffusion method. RESULTS The optimal extraction process of G. littoralis was solid-liquid ratio of 1∶15， distillation time of 5 hours， and KCl concentration of 15%. Eleven components were identified from the volatile oil of the stems and leaves of G. littoralis， and a total of eight components were identified from the volatile oil of the roots. Ginsenethinol was a common component in the volatile oil from the stems， leaves and roots of G. littoralis， its contents in the stems and leaves， roots were 38.21% and 74.02%， respectively. The volatile oil from the stems， leaves and roots of G. littoralis had a certain E-mail：zwhjzs@126.com inhibitory effect on F. solani， F. incarnatum， F. oxysporum， A. parasiticus and A. flavus， especially volatile oil from the stems and leaves. CONCLUSIONS There is a significant difference in chemical components of the volatile oil between the roots， stems and leaves of G. littoralis， both of which have certain in vitro antifungal activity.
