Simultaneous determination of 8 components in Jinqi jiangtang tablets by ion mobility mass spectrometry method
- VernacularTitle:离子淌度质谱法同时测定金芪降糖片中8种成分含量
- Author:
Yingcai LI
1
;
Hecheng HUANG
2
;
Ye YANG
3
Author Information
1. Kunming Institute of Food and Drug Control，Kunming 650032，China
2. Yunnan Fair Quality Inspection Co.，Ltd.，Kunming 650214，China
3. School of Life Sciences and Technology，Kunming University of Science and Technology，Kunming 650500，China
- Publication Type:Journal Article
- Keywords:
ion mobility mass spectrometry method;
Jinqi jiangtang tablets;
active ingredient;
content determination
- From:
China Pharmacy
2023;34(11):1354-1357
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish the ion mobility mass spectrometry method for simultaneous determination of epiberberine， berberine， coptisine， palmatine， calycosin-7-glucoside， 3，5-O-dicaffeoylquinic acid， 4，5-O-dicaffeoylquinic acid and chlorogenic acid in Jinqi jiangtang tablets. METHODS Ion mobility mass spectrometry method was used. The determination was performed on Waters ACQUITY UPLC HSS T3 （2.1 mm×50 mm， 1.8 μm） with mobile phase consisted of 0.1% formic acid solution-acetonitrile （gradient elution） at the flow rate of 0.3 mL/min. The column temperature was 40 ℃， and the injection volume was 5 μL. The contents of 8 components in Jinqi jiangtang tablets were determined by scanning detection under positive and negative ion modes with an electric spray ion source， and setting ion mobility mass parameters according to the peak response of each component. RESULTS The results showed that the linear relationship of the eight components was good within their respective ranges （r≥0.999）； RSDs of precision， repeatability and stability （24 h） tests were not more than 4.0%； average recoveries were 94.6%-101.2% ， RSDs were 2.6%-3.9% （n＝9）. The contents of the above eight components in three batches of Jinqi jiangtang tablets were 3.060-3.545， 24.50-26.74， 2.795-4.149， 1.437-2.501， 0.204-0.242， 0.950-1.281， 2.272-2.828， 7.314- 7.960 mg/g， respectively. CONCLUSIONS The established method has high sensitivity and good reproducibility， and can provide reference for the quality control of the preparation.
