Knockdown of ClC-2 Gene Expression Inhibits the Growth of BT-325 Human Glioma Cells
- VernacularTitle:干扰ClC-2基因表达对胶质瘤BT-325细胞生长的影响
- Author:
Xiang-Yun YANG
;
Xiao-Gang LAI
;
Yong ZHANG
;
Jianming PEI
;
Angang YANG
;
Shisheng ZHOU
- Publication Type:Journal Article
- Keywords:
ClC-2 gene, glioma, RNA interference (RNAi), BT-325 cells
- From:
Chinese Journal of Rehabilitation Theory and Practice
2006;12(5):378-380
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the growth of BT-325 human glioma cells after interfering volume-regulated chloride channel ClC-2 gene.MethodsTwo expression recombinant vectors of ClC-2 gene were designed and constructed. The primary plasmid, pSUPER.puro-shRNA, and the two recombinant plasmids, pSUPER.puro-shRNA-ClC-21 and pSUPER.puro-shRNA-ClC-22, were transfected into BT-325 cells by LipofectamineTM2000 (Groups: control, PP1 and PP2, respectively). The mRNA expression of ClC-2 gene was detected with reverse transcription polymerasse chain reaction (RT-PCR), the cellular survival was determined with MTT assay, and the cell cycle was measured with flow cytometry (FCM). ResultsClC-2 mRNA expression and the growth of the cells in PP1 and PP2 groups were significantly lower than that of control group. The cell cycle progression was blocked in G1 phase (PP1 and PP2 vs control,P<0.01). ConclusionThe growth of BT-325 human glioma cells is prevented by knockdown of ClC-2 gene expression, which may be one of the novel targets to inhibit growth of human malignant glioma cells.
