Transfection of Nitric Oxide Synthase Gene with Adenovirus Can Inhibit the Intimal Hyperplasia of Venous Autografts
- VernacularTitle:腺病毒转染一氧化氮合酶基因抑制大鼠静脉桥血管内膜增生
- Author:
Tie-ling LI
;
Jin-shan ZHANG
;
Shui-long GUO
;
Li FAN
- Publication Type:Journal Article
- Keywords:
nitric oxide synthase (NOS), restenosis, vascular grafts, gene therapy
- From:
Chinese Journal of Rehabilitation Theory and Practice
2006;12(5):376-377
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effects of nitric oxide synthase (NOS) genetic transfection on the intimal hyperplasia of venous autografts. MethodsThe external jugular veins were autografted into abdominal aorta arteries in 20 Wistar rats, which were divided evenly into experimental or control groups. The transplanted veins of experimental group were immersed in the adenovirus-mediated eNOS gene solution for 15 minutes just before anastomosis. The transplanted vascular samples were taken out 2 weeks after operation. The intimal thickness(IH), degree of restenosis(DR), expression of PCNA and NOS mRNA were determined with histology and transcription polymerase chain reaction (PCR). ResultsThe IH, DR and PCNA decreased, while the expression of eNOS mRNA increased comparing with control group(P<0.01). ConclusionTransfection of NOS gene can inhibit the intimal hyperplasia of venous autografts.
