Effect of Extracorporeal Shock Wave on Proliferating Activity of Human Mesenchymal Stem Cells
- VernacularTitle:体外冲击波干预对成人骨髓间充质干细胞增殖活性的影响
- Author:
Wu-zhou WANG
;
Geng-yan XING
;
Ru-fang JING
;
Zhengda KUANG
;
Chuan JIANG
;
Anquan ZHOU
;
Zengqian YANG
- Publication Type:Journal Article
- Keywords:
extracorporeal shock wave, human mesenchymal stem cells, proliferation
- From:
Chinese Journal of Rehabilitation Theory and Practice
2006;12(5):372-375
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the mechanism of extracorporeal shock wave (ESW) in treating osteogenic disorders and the ideal energy level. MethodsAfter success in marrow aspiration from patients' iliac crest, hMSCs were isolated by Percoll density gradient centrifugation and cultured in Dulbecco's modified Eagle's medium in a 5% CO2 and 37 ℃ incubator. Optimal ESW dose was determined by MTT of kinase-marked cytobiology. After hMSCs were exposed to ESW, their morphocytologic change, rate of adherence and doubling time were observed with IPCM. Enzyme cytochemistry reaction for the activity of alkaline phosphatase was also examined. ResultsESW of 5 kV and 100 times could increase cells' viability and proliferation (P<0.01), but higher than 7 kV would inhibit them. Rate of adherence of hMSCs in exposure group of passage 5 reached to 61.54%, which was significantly different from control group(P<0.05). Compared with control group, the MSCs' doubling time was short for 1.72 d (P<0.05). The curve of normal alkaline phosphatase activity of hMSCs was like type S, but ESW shortened its latent period, and promoted its peak time, which was significantly different from control group.ConclusionESW of 5 kV and 100 times can optimally promote the proliferation and activity of osteogen of hMSCs in vitro.
