Inhibition of Pingliukang Medicated Serum on Proliferation of Rat C6 Glioma Cells in Vitro
- VernacularTitle:平瘤康方剂对C6胶质瘤细胞增殖的抑制作用
- Author:
Zhenfei LI
;
Haikui ZHANG
;
Xiaoming ZHANG
;
Ling YIN
- Publication Type:Journal Article
- Keywords:
Pingliukang prescription, serum pharmacology, C6 glioma cell, proliferation, cell cycle
- From:
Chinese Journal of Rehabilitation Theory and Practice
2007;13(5):438-439
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitory effect of Pingliukang (a prescription) medicated serum on the proliferation of cultured rat C6 glioma cells and influence on cell cycle in vitro. Methods MTT colorimetry were performed to measure the levels of the proliferation of rat C6 glioma cells cultured with 2.5%, 5%, 10% and 20% of Pingliukang medicated serum for 24 h, 48 h and 72 h in vitro. The effect of Pingliukang medicated serum on cell cycle were observed by FCM. Results The proliferation of C6 cell was obviously inhibited by Pingliukang medicated serum with dose-effect relationship. The inhibitory effect of 20% of medicated serum was the strongest. When the C6 glioma cells were treated with 10% and 20% medicated serum for 48 h and 72 h, the cells of S period declined. Conclusion The Pingliukang medicated serum can inhibit the proliferation and block cell cycle of cultured C6 glioma cells.
