Construction of Recombinant Eukaryotic Expression Vector for Human Endostatin and Its Expression in HEK293 Cells
- VernacularTitle:分泌人内皮抑素基因工程细胞系的建立
- Author:
Hui ZHAO
;
Jingkun PAN
;
Yun LUO
;
Yilong XUE
- Publication Type:Journal Article
- Keywords:
endostatin, eukaryotic expression vector, HEK293, cell line
- From:
Chinese Journal of Rehabilitation Theory and Practice
2007;13(5):422-424
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct a cell line with long time secreting endostatin. Methods Human endostatin cDNA containing interleukin-2 (IL-2) secreting peptide was cloned into eukaryotic expression plasmid pSNA2.0 to construct recombinant plasmid pSNA2.0/Endostatin. The plasmid pSNA2.0/Endostatin was transfected into HEK293 cells by cationic liposome. The positive cell clones were selected by G418 and named hED/293. The expression of endostatin protein was analyzed by Western-blot. Results Endostatin could be determined in the supernatant of hED/293 cells. Conclusion The recombinant eukaryotic expression vector is correctly constructed. The human endostatin protein can be expressed and secreted.
