Effect of the Jiedu Huayu Ⅱ Decoction on Mitochondrial Bcl-2 and Cytochrome C Expression in Liver in Rats with Acute Hepatic Failure
- VernacularTitle:解毒化瘀Ⅱ方对急性肝衰竭大鼠肝细胞Bcl-2及细胞色素C表达的影响
- Author:
Dewen MAO
;
Hua QIU
;
Chaozan NONG
;
Jie LIU
;
Qinglan SHI
- Publication Type:Journal Article
- Keywords:
Jiedu Huayu Ⅱ Decoction, acute hepatic failure, mitochondria, Bcl-2, cytochrome C, rats
- From:
Chinese Journal of Rehabilitation Theory and Practice
2007;13(3):255-257
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of the Jiedu Huayu Ⅱ Decoction in actue hepatic failure.MethodsThe acute hepatic failure rat models were induced with thioacetamide (TAA).84 SPF Wistar rats were divided into 7 groups randomly:blank group,model group,low dose group,middle dose group,high dose group,lactulose group,and the Angong Niuhuang Pill group.The drugs were administrated by gavage 3 d before model-making,5.5 d in total.The expression of Bcl-2,cytochrome C in mitochondrial and cytoplasm were measured with Western Blot.ResultsThe expression of Bcl-2 reduced in mitochondrial,the expression of cytochrome C increased in cytoplasm and reduce in mitochondrial in model group compare with that of blank group.Jiedu Huayu Ⅱ Decoction was able to enhance the expression of Bcl-2 and restrain cytochrome C release from mitochondrial to cytoplasm.Moreover,it shows dose-effect relation.ConclusionThe protection of Jiedu Huayu Ⅱ Decoction for hepatocyte in acute hepatic failure rats maybe relate to the increase of Bcl-2 in mitochondrial,blocking the mitochondrial permeability transition pore to arrest cytochrome C releasing.
