Effect of Intrathymic Injection of Allogene Bone Marrow on Small Intestine Transplantation of Rats
- VernacularTitle:骨髓细胞胸腺内注射对大鼠小肠移植急性排斥反应的影响
- Author:
Chang-jiang LUO
;
Wei-zhong WANG
;
Ji-peng LI
;
Dongli CHEN
;
Guanglong DONG
;
Ji LIU
- Publication Type:Journal Article
- Keywords:
intrathymic injection, bone marrow cell, small intestine transplantation, immune tolerance, soluble interleukin-2 receptor (sIL-2R), tumor necrotic factor-α (TNF-α)
- From:
Chinese Journal of Rehabilitation Theory and Practice
2006;12(4):304-306
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the immune tolerance function and significance of allogene bone marrow injection to the small intestines transplantation of rats.MethodsInbreeding line rat F344/N and Wistar/A were selected to perform heterotopic graft of the whole small intestine. 7 days before allogene transplantation, donator bone marrow cells (BMC) were injected into thymus of acceptor (the testing group). According to the isogene and allogene rat transplant model, it was comprehended whether injecting allogene donator marrow into acceptor thymus could decrease the acute rejection after transplantation.Results3, 5 or 7 days after allogeneic rat dystopia whole small intestine transplantation, typical reject reaction appeared, but there was no reject reaction in isogenome and testing group. 3 days after allotransplantation, serum soluble interleukin-2 receptor (sIL-2R) and tumor necrotic factor-α (TNF-α) levels were significantly higher than the other groups (P<0.01). The level of serum sIL-2R and TNF-α in the allogene marrow injecting group were only slight higher on the 3rd or 5th day, and getting downtrend, and there was no significant difference compared with isogenic transplantation group.ConclusionAllogenic donator bone marrow intrathymic injecting into acceptor 7 days before small intestina transplantation, can reduce the reject reaction after the grafting. The levels of serum sIL-2R and TNF-α can be selected as a sensitive early diagnosis index of acute rejection after small intestine transplantation.
