Protective Effect of Heat Shock Protein 27 on Cardiomyocytes when Ischemic Preconditioning Performed in Rat
- VernacularTitle:HSP27在大鼠心肌细胞缺氧预处理中的保护作用及其机制
- Author:
Xue-song ZHANG
;
Xue-yan ZHANG
;
Xiujuan YANG
;
Hong GUO
;
Xianfeng XIN
;
Fanrong ZENG
;
Huiming ZUO
- Publication Type:Journal Article
- Keywords:
heat shock protein 27 (HSP27), ischemic preconditioning, cardiomyocyte, apoptosis, caspase-3
- From:
Chinese Journal of Rehabilitation Theory and Practice
2006;12(4):301-303
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the mechanism and the protective effect of heat shock protein 27 (HSP27) on rat cardiomyocytes when ischemic preconditioning performed.MethodsCultured rat cardiomyocytes were divided into four groups: control group, ischemic group,ischemic preconditioning group and cyclohexamide group. Cell viabilities were analyzed by MTT. The apoptosis was evaluated with DNA ladder and flow cytometry Annexin V Flous staining. Western Blot was used to determine the expression of HSP27 and caspase-3 in cardiomyocytes.ResultsIschemic preconditioning could improve cell viability. The apoptosis ratio in ischemic preconditioning group was significantly less than that in ischemic group. These were accompanied by an increase in the expression of HSP27 and a decrease in caspase-3. The expression of the increased HSP27 and the protective effect induced by ischemic preconditioning were completely abolished by the presence of cycloheximide, a translation inhibitor.ConclusionThe expression of HSP27 induced by ischemic preconditioning plays an important role in protecting cardiomyocytes, and the mechanism is possibly related to the inhibition of cell apoptosis.
