Advances in loop-mediated isothermal amplification in the diagnosis of tuberculosis
10.13604/j.cnki.46-1064/r.2022.11.19
- Author:
LUO Li-sha
;
LIU Lin
;
FENG Pin
;
LAI Ji-jia
;
CHEN Xue-yuan
;
KONG Qing-quan
- Publication Type:Journal Article
- Keywords:
Loop-mediated isothermal amplification;
tuberculosis;
diagnosis
- From:
China Tropical Medicine
2022;22(11):1097-
- CountryChina
- Language:Chinese
-
Abstract:
Abstract: The loop-mediated isothermal amplification (LAMP) technique is a technique for the specific and efficient amplification of target fragments at a constant temperature using two pairs of specially designed primers and a strand displacement activity DNA polymerase. LAMP technique is a simple, rapid, specific, sensitive and cost-effective nucleic acid amplification method, and therefore has a promising future in the field rapid detection of Mycobacterium tuberculosis and grassroots applications. In this review, the basic principles and characteristics of the LAMP technique, the main molecular markers for the diagnosis of tuberculosis, and the use of different molecular markers and various types of novel techniques in the diagnosis of pulmonary tuberculosis, extrapulmonary tuberculosis, and drug-resistant tuberculosis were described. The LAMP technique has been widely used in the diagnosis of tuberculosis with high sensitivity and specificity, but the technique still has some shortcomings. This paper reviews the progress of its application in tuberculosis in recent years and provides an outlook on its development, with a view to providing a rational research direction for rapid diagnosis of tuberculosis in a resource-limited environment.
- Full text:19.Advances in loop-mediated isothermal amplification in the diagnosis of tuberculosis.pdf
