Biocontrol capability of bacteriophages against soft rot disease caused by Dickeya chrysanthemi

Jia Rou Khor; Sasireigga Jaya Jothi; Sinniah Kurunathan; Ke Yuh Lee; Su Yin Lee; Siok-yee Chan

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Biocontrol capability of bacteriophages against soft rot disease caused by Dickeya chrysanthemi

Author: Jia Rou Khor ^{1
,

2} ; Sasireigga Jaya Jothi ³ ; Sinniah Kurunathan ⁴ ; Ke Yuh Lee ⁴ ; Su Yin Lee ^{2
,

5} ; Siok-Yee Chan ⁶
Author Information

1. School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800, Pulau Pinang, Malaysia. &
2. Department of Biotechnology, Faculty of Applied Sciences, AIMST University, 08100, Semeling, Kedah, Malaysia.
3. Centre of Excellence for Omics-Driven Computational Biodiscovery (COMBio), Faculty of Applied Sciences, AIMST University, 08100, Semeling, Kedah, Malaysia.
4. Department of Biotechnology, Faculty of Applied Sciences, AIMST University, 08100, Semeling, Kedah, Malaysia.
5. Centre of Excellence for Omics-Driven Computational Biodiscovery (COMBio), Faculty of Applied Sciences, AIMST University, 08100, Semeling, Kedah, Malaysia. &
6. School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800, Pulau Pinang, Malaysia.
Publication Type:Journal Article
Keywords: Bacteriophage; Dickeya chrysanthemi; Soft rot disease; Phage treatment
MeSH: Bacteriophages--isolation & purification; Dickeya chrysanthemi
From:Malaysian Journal of Microbiology 2021;17(6):615-623
CountryMalaysia
Language:English
Abstract: Aims:This study aims to evaluate the effectiveness of bacteriophages isolated from Klang and Penang, Malaysia against Dickeya chrysanthemi that causes soft rot disease.
Methodology and results:Basic characterization such as dextrose test, citrate test, lactose fermentation test and ornithine test were carried out on D. chrysanthemi. Activity of bacteriophages against D. chrysanthemi was evaluated using spot test. Double agar overlay assay was performed to purify and enumerate the quantify of bacteriophages.Bacteriophages were also checked for its effectiveness in controlling soft rot on post-harvested vegetables: potato (Solanum tuberosum), cucumber (Cucumis sativus) and apple (Malus domestica). Results showed that D. chrysanthemiable to utilize citrate and dextrose as the source of energy, which indicated that D. chrysanthemi inclined to choose fruits and vegetables containing citrate and dextrose as the target of attack. Clear zone observed on the bacterial lawn (spot test) indicated the ability of the bacteriophages to infect and lyse D. chrysanthemi. All the bacteriophages studied herein reached the highest concentration on day 3 and were monovalent.
Conclusion, significance and impact of study:All the isolated bacteriophages were able to restrain the spreading of soft rot caused by D. chrysanthemi either work alone or as cocktail. This study provides information for the formulation development of bacteriophage against soft rot disease cause by D. chrysanthemi. Furthermore, this study reveals the potential of locally isolated bacteriophages against the D. chrysanthemi and paving the application of phage treatment on agriculture products that are not limited to potatoes, cucumber and apple.
Full text:20.2021my0062.pdf