Hepatocyte nuclear factor 1-alpha mutation in normal glucose-tolerant subjects and early-onset type 2 diabetic patients.
10.3904/kjim.2008.23.4.165
- Author:
Dong Mee LIM
1
;
Nam HUH
;
Keun Yong PARK
Author Information
1. Department of Internal Medicine, Konyang University College of Medicine, Daejon, Korea. kypark@kyuh.co.kr
- Publication Type:Original Article
- Keywords:
DNA chips;
MODY;
Type 2 diabetes mellitus
- MeSH:
Adolescent;
Adult;
Age of Onset;
Asian Continental Ancestry Group/genetics/*statistics & numerical data;
Case-Control Studies;
Cohort Studies;
Diabetes Mellitus, Type 2/*epidemiology/*genetics;
Female;
Hepatocyte Nuclear Factor 1-alpha/*genetics;
Humans;
Korea;
Male;
Mutation/*genetics;
Prevalence;
Young Adult
- From:The Korean Journal of Internal Medicine
2008;23(4):165-169
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND/AIMS: The prevalence of diabetes in Korea is reported to be approximately 10%, but cases of maturity-onset diabetes of the young (MODY) are rare in Korea. A diagnostic technique for autosomal dominant MODY is being actively sought. In this regard, we used a DNA chip to investigate the frequency of mutations of the MODY3 gene (hepatocyte nuclear factor-1alpha) in Korean patients with early-onset type 2 diabetes. METHODS: The genomic DNA of 30 normal individuals [age, 24.9+/-8.6 years] and 25 patients with early-onset type 2 diabetes (age, 27+/-5.9 years) was extracted, and the MODY3 gene was amplified. The amplified DNA was hybridized onto a MODY3 chip, which has oligonucleotides of 15-25 bases, representing wild-type and mutant MODY3 sequences in both forward and reverse orientations, immobilized on its surface. RESULTS: Among the normal subjects, there was no mutation of MODY3. Among those with early-onset type 2 diabetes, there was one case of MODY3 mutation. CONCLUSIONS: Our results indicate that MODY3 mutations are not rare in Korean early-onset type 2 diabetes patients in Korea and suggest that MODY3 mutations in patients with early-onset type 2 diabetes need to be further evaluated.
