Antifungal effects and phytochemical screening of Andrographis paniculata extracts on dermatomycoses

Tuan Kub Tuan Noorkorina; Farhaana Mohd Ab Aziz; Anis Amiera Muhamad Alojid; Nursaadatun Nisak Ahmad; Zeti Nurfidiyati Salmuna; Siti Asma&rsquo; Hassan; Sabarisah Hashim; Azian Harun

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Antifungal effects and phytochemical screening of Andrographis paniculata extracts on dermatomycoses

Author: Tuan Kub Tuan Noorkorina ^{1
,

2} ; Farhaana Mohd Ab Aziz ³ ; Anis Amiera Muhamad Alojid ³ ; Nursaadatun Nisak Ahmad ⁴ ; Zeti Nurfidiyati Salmuna ^{1
,

2} ; Siti Asma’ Hassan ^{1
,

2} ; Sabarisah Hashim ⁵ ; Azian Harun ^{1
,

2}
Author Information

1. Department of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia. &
2. Medical Microbiology and Parasitology Laboratory, Hospital Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia.
3. Department of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia.
4. Faculty of Business and Management, Universiti Teknologi Mara, Puncak Alam, Bandar Puncak Lama, 42300 Kuala Selangor, Selangor, Malaysia.
5. Department of Neuroscience, School of Medical Sciences, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia.
Publication Type:Journal Article
Keywords: Andrographis paniculata; Antifungal; Mycelial inhibition; Phytochemical; Spore
MeSH: Antifungal Agents; Phytochemicals; Andrographis paniculata; Dermatomycoses
From:Malaysian Journal of Microbiology 2021;17(5):576-587
CountryMalaysia
Language:English
Abstract: Aims:Andrographis paniculata (AP), a medicinal herb was selected to investigate the antifungal activity on selected dermatophyte fungi. The phytochemical screening was also carried out to evaluate its chemical constituents.
Methodology and results:The potato dextrose agar (PDA) incorporated with aqueous, ethanol and methanol AP extracts at concentrations 0.99% (v/v), 1.96% (v/v) and 7.41% (v/v) were used for selected fungi culturing; Trichophyton mentagrophytes, T. rubrum, T. interdigitale, Microsporum fulvum, M. nanum, M. gypseum, M. canis, Fusarium solani and Aspergillus fumigatus. Phytochemical screening showed the presence of flavonoids, saponins and tannins in the ethanol extract and flavonoids alone in both aqueous and methanol extracts. Studies on antifungal effects indicated that the ethanol extract significantly increased the mycelial inhibition percentage of all tested fungi, especially at a concentration of 7.41% (v/v). All ethanol AP extract concentrations inhibited M. gypseum and M. canis (p<0.05) with at least 36.00% mycelial inhibition. In aqueous AP extract, it significantly increased the mycelial inhibition of T. mentagrophytes, T. interdigitale and M. gypseum (p<0.05), while the methanol AP extract significantly inhibited all fungi at a concentration of 7.41% (v/v) except for T. rubrum, M. gypseum and F. solani (p<0.05). No spore sedimentation was recorded for the fungal spores of T. rubrum, M. nanum, T. mentagrophytes, M. gypseum and T. interdigitale at 7.41% (v/v) ethanol AP.
Conclusion, significance and impact of study:It is concluded that the ethanol AP extract contained phytochemical constituents and showed the highest antifungal activity. In addition, this extract has a great potential to treat dermatophytes effectively.
Full text:20.2021my0058.pdf