The effect of electroporation on the growth characteristics and antimicrobial activity of lactic acid bacteria

Howe-nguong Wong; Chee-sian Kuan; Chee-hao Kuan; Siok-koon Yeo

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The effect of electroporation on the growth characteristics and antimicrobial activity of lactic acid bacteria

Author: Howe-Nguong Wong ¹ ; Chee-Sian Kuan ² ; Chee-Hao Kuan ³ ; Siok-Koon Yeo ¹
Author Information

1. School of Biosciences, Taylor(s) University Lakeside Campus, No 1, Jalan Taylor(s), 47500 Subang Jaya, Selangor Darul Ehsan, Malaysia.
2. Neogenix Laboratoire Sdn Bhd, C707, Level 7, Block C, Kelana Square, Kelana Jaya, 47301 Petaling Jaya, Selangor, Malaysia.
3. Department of Food Science with Nutrition, Faculty of Applied Sciences, UCSI University, No. 1, Jalan Menara Gading, UCSI Heights, 56000 Cheras, Wilayah Persekutuan Kuala Lumpur, Malaysia.
Publication Type:Journal Article
Keywords: Lactic acid bacteria; Bacteriocin; Electroporation; Growth; Antimicrobial
MeSH: Electroporation; Lactobacillales
From:Malaysian Journal of Microbiology 2021;17(5):514-524
CountryMalaysia
Language:English
Abstract: Aims:This study aimed to evaluate the effect of electroporation on the growth characteristics and antimicrobial activity of lactic acid bacteria (LAB) including Bifidobacterium longum ATCC 15707, Lactobacillus acidophilus ATCC 314, Lactobacillus casei ATCC 393 and Lactobacillus fermentum ATCC 14931.
Methodology and results: Electroporation with the strength of electric field at 1.0–3.0 kV/cm for 2-4 millisecond were applied on the bacterial cultures. All bacterial cultures showed significant (P<0.05) increased in cell viability (40%-325%) upon electroporation. Such treatment also increased the acidity of the cell where the pH of cells decreased upon treatment. In tandem with the increased viability, electroporated bacterial cultures also showed higher proteolytic activity compared to the control (P<0.05). The electroporation treatment also increased (P<0.05) the bacteriocin activity of treated cells compared to the control. However, the molecular weight of bacteriocins produced were not affected by electroporation. Treated cells also possessed better antimicrobial activity. According to the results collected, all treated LAB strains showed 11.5%-113.8% higher (P<0.05) inhibitory activity compared to untreated control against tested pathogenic bacteria, Escherichia coli and Listeria monocytogenes that commonly associated with food contamination. Microarray data analysis showed that electroporation regulated the entities encoding for surface protein and transporter.
Conclusion, significance and impact of study:The results from this study suggested that electroporation could enhance the growth characteristics and antimicrobial activity of LAB by modifying the surface regions of the cells. This result may serve as the reference for food manufacturers to opt for effective biopreservation method and produce food with extended shelf life.
Full text:20.2021my0053.pdf