The effect of X-ray on microglial M1 and M2 polarization

Rongrong Huang; Yan Zhou; Ling Guo; Liyuan Liu; Yizhe Xue; Panpan Lai; Yuntao Jing; Hui XU; Qinfeng Zhang; Guirong Ding

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The effect of X-ray on microglial M1 and M2 polarization

VernacularTitle:X 射线对小胶质细胞 M1 和 M2 型极化的影响
Author: Rongrong HUANG ^{1
,

2} ; Yan ZHOU ³ ; Ling GUO ³ ; Liyuan LIU ³ ; Yizhe XUE ³ ; Panpan LAI ³ ; Yuntao JING ³ ; Hui XU ⁴ ; Qinfeng ZHANG ⁵ ; Guirong DING ³
Author Information

1.
2. School of Public Health, Shandong First Medical University/Department of Radiation Protec- tion Medicine, Department of Military Preventive Medicine, Air Force Medical University
3. Department of Radiation Protec- tion Medicine, Department of Military Preventive Medicine, Air Force Medical University
4. College of Radiology, Shandong First Medical University
5. School of Public Health, Shandong First Medical University
Publication Type:Journal Article
Keywords: Ionizing Radiation; X-ray; Microglia Cells; M1 Polarization; M2 Polarization
From: Chinese Journal of Radiological Health 2021;30(3):247-252
CountryChina
Language:Chinese
Abstract: Objective:To investigate the effect of X-ray on the polarization of mouse microglia BV-2 cells.
Methods:BV-2 cells at the logarithmic growth stage were randomly divided into the Sham irradiation group and 10 Gy irradiation group. The latter group was given a single X-ray irradiation at a dose of 1.28 Gy/min for 7 min 49 s. The activation rate of BV-2 cells was observed and analyzed under a microscope at 1, 3, 6, 24 h and 48 h after irradiation.The changes of cell morphology were observed by HE staining and immunofluorescence staining; The levels of M1-type activation markers (TNF-α and IL-1β) and M2-type activation marker TGF-β1 in the supernatant of BV-2 cells were detected by ELISA. The levels of polarization-related proteins of M1-type (CD86 and iNOS) and M2-type (CD206) in BV-2 cells were detected by Western blotting.
Results　:Morphological results showed that BV-2 cells became larger, and their protrude became coarse and shorter, showing "amoeba" like changes after 10 Gy X-ray irradiation. Compared with the Sham group, the activation rate of BV-2 cells was significantly increased at 3 h, and reached the peak at 6 h, and began to recover at 48 h after irradiation. ELISA results showed an obvious increase in the level of TNF-α and TGF-β1 48 h after irradiation.The level of IL-1β showed a transient decrease at 3～6 h, increased at 24 h, and reached the peak 48 h after irradiation. Western blotting results showed that CD86 protein level did not change significantly at each time points after irradiation, and iNOS protein level in- creased significantly at 1, 6, 24 h and 48 h after irradiation. A fluctuating change in CD206 protein level was found after irradiation.
Conclusion　:10 Gy X-ray irradiation can induce the activation of BV-2 cells in vitro, and the polarization type changes with the time after irradiation.