Biological Mechanism of Drought Improving Quality of Rhizoma Atractylodis Chinensis
10.13422/j.cnki.syfjx.20230514
- VernacularTitle:干旱提高北苍术药材质量的生物学机制
- Author:
Kai ZHAO
1
;
Jie YAO
1
;
Pengcheng YU
1
;
Xiaowen SONG
1
;
Yao YAO
1
;
Luwen HE
1
;
Xiangcai MENG
1
Author Information
1. Heilongjiang University of Chinese Medicine，Harbin150040， China
- Publication Type:Journal Article
- Keywords:
Atractylodes chinensis;
drought stress;
reactive oxygen species;
secondary metabolites;
quality of medicinal herbs
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2023;29(13):180-187
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the biological mechanism of drought improving the quality of Rhizoma Atractylodis Chinensis and establish a new method for the production of high-quality medicinal materials. MethodThe fresh roots of Atractylodes chinensis were soaked in 0 （control）， 5%， 10%， and 20% PEG-6000 solutions. The changes in reactive oxygen species （ROS） level， antioxidant enzyme activity， activities of key enzymes in primary metabolism and secondary metabolism， and content of secondary metabolites were compared. ResultCompared with the control group， the treatment with 20% PEG for 2 days elevated the levels of superoxide anion radicals （O2-
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·）， hydrogen peroxide （H2O2）， and malondialdehyde （MDA） by 172.5%， 56.9%， and 14.7%， respectively. The treatment did not change the activity of superoxide dismutase （SOD）， reduced the peroxidase （POD） activity， and increased the catalase （CAT） activity by 10.8%. It increased the activities of 3-hydroxy-3-methylglutaryl-CoA reductase （HMGR）， phosphoenolpyruvate carboxylase （PEPC）， and acetyl-CoA carboxylase （ACC） by 49.9%， 12.1%， and 19.0%， respectively. Furthermore， the content of atractylodin， β-eudesmol， atractylone， and atractylenolide Ⅱ was increased by 51.0%， 36.9%， 47.1%， and 91.5%， respectively. The simulated drought stress can cause the burst of ROS in the fresh roots of A. chinensis， induce the physiological state of plants under drought， change the antioxidant system， and promote the massive synthesis of secondary metabolites in a short time. ConclusionPEG-6000-simulated drought stress can greatly improve the quality of A. chinensis in cultivation.
