Quality Marker （Q-marker） of Tinosporae Radix Associated with Efficacy of "Relieving Sore Throat"
10.13422/j.cnki.syfjx.20230117
- VernacularTitle:“利咽”功效关联的金果榄质量标志物分析
- Author:
Lijie LU
1
;
Qinghua WU
1
;
Xinglong ZHU
1
;
Xulong HUANG
1
;
Huanan RAO
1
;
Bin XIAN
1
;
Feiyan WEN
1
;
Tao ZHOU
1
;
Min WEI
2
;
Sanbo LIU
3
;
Jin PEI
1
Author Information
1. School of Pharmacy， State Key Laboratory of Southwestern Chinese Medicine Resource， Chengdu University of Traditional Chinese Medicine（TCM），Chengdu 611137，China
2. China Resources Sanjiu Medical ＆ Pharmaceutical Co. Ltd.， Shenzhen 518110， China
3. China Resources Sanjiu （Huangshi） Pharmaceutical Co. Ltd.， Huangshi 435003， China
- Publication Type:Journal Article
- Keywords:
Tinosporae Radix;
relieving sore throat;
ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry （UPLC-Q-TOF-MS）;
multivariate statistical analysis;
network pharmacology;
quality marker
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2023;29(13):140-150
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the potential quality marker （Q-marker） of Tinosporae Radix associated with efficacy of "relieving sore throat" based on ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry （UPLC-Q-TOF-MS）， multivariate statistical analysis （MSA）， and network pharmacology. MethodUPLC-Q-TOF-MS was used to identify the main chemical components in 18 batches of Tinosporae Radix. On this basis， principal component analysis （PCA） and orthogonal partial least squares-discriminant analysis （OPLS-DA） were employed to screen out the main marker components that caused differences between groups. Moreover， network pharmacology technology was applied to predict the potential "sore throat-relieving" components， and the molecular docking between the common components resulting from MSA and network pharmacology and the core targets was carried out to verify the marker components. ResultA total of 17 compounds， including alkaloids， diterpenoid lactones， and sterols， were identified by UPLC-Q-TOF-MS. Five main differential components were found by MSA： Columbamine， jatrorrhizine， palmatine， menisperine， and columbin. Network pharmacology analysis yielded six compounds： tetrahydropalmatine， palmatine， menisperine， fibleucin， neoechinulin A， and columbin which were selected as potential "sore throat-relieving" components of Tinosporae Radix. They may relieve sore throat by acting on interleukin-6， epidermal growth factor receptor， prostaglandin G/H synthase 2， matrix metalloproteinase-9， proto-oncogene tyrosine-protein kinase Src and other targets， and regulating Hepatitis B， influenza A， human T-cell virus infection， human cytomegalovirus infection， coronavirus disease-2019， and other signaling pathways. The common active components in Tinosporae Radix resulting from MSA and network pharmacology analysis were palmatine， menisperine， and columbin， which had high binding affinity with six core targets and can be used as the Q-marker components of Tinosporae Radix in "relieving sore throat". ConclusionThis study predicts the "sore throat-relieving" Q-marker of Tinosporae Radix， which lays a basis for developing the quality standard of Tinosporae Radix based on the efficacy and improving the quality evaluation system of the medicinal.
