Analysis on Quality Standard of Fraxini Cortex（Fraxinus chinensis） Dispensing Granules Based on Standard Decoction
10.13422/j.cnki.syfjx.20230148
- VernacularTitle:基于标准汤剂的秦皮（白蜡树）配方颗粒质量标准分析
- Author:
Guiyun CAO
1
;
Bo NING
1
;
Jinmiao QIN
1
;
Xuesong ZHUANG
1
;
Daijie WANG
2
;
Yongqiang LIN
3
;
Xiaodi DONG
1
;
Yi LUO
1
;
Zhaoqing MENG
1
Author Information
1. Shandong Province Technical Innovation Center of Traditional Chinese Medicine Treatment of Respiratory Diseases，Shandong Hongjitang Pharmaceutical Group Co. Ltd.，Jinan 250103，China
2. Shandong Analysis and Test Center，Qilu University of Technology（Shandong Academy of Sciences），Jinan 250014，China
3. Shandong Institute for Food and Drug Control，Jinan 250101，China
- Publication Type:Journal Article
- Keywords:
Fraxini Cortex;
standard decoction;
dispensing granules;
quantitative analysis of multi-components by single marker（QAMS）;
specific chromatograms;
quality control;
high performance liquid chromatography（HPLC）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2023;29(13):122-129
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo establish the quality standard for Fraxini Cortex（Fraxinus chinensis） dispensing granules based on standard decoction， and to provide a basis for the quality control of this dispensing granules. MethodHigh performance liquid chromatography（HPLC） specific chromatograms of 15 batches of Fraxini Cortex（F. chinensis） standard decoctions and 3 batches of Fraxini Cortex（F. chinensis） dispensing granules were established with the mobile phase of 0.1% phosphoric acid aqueous solution（A）-acetonitrile（B） for gradient elution（0-10 min， 12%-15%B； 10-30 min， 15%-32%B） and the detection wavelength of 220 nm. And similarity evaluation， cluster analysis and principal component analysis（PCA） were also carried out. HPLC quantitative analysis of multi-components by single marker（QAMS） was established to determine the contents of the main components in the standard decoctions and dispensing granules. The contents of the corresponding components in Fraxini Cortex（F. chinensis） decoction pieces were also detected， and the transfer rates from decoction pieces to standard decoctions and dispensing granules were calculated. ResultThe similarities between specific chromatograms of 15 batches of Fraxini Cortex（F. chinensis） standard decoctions and 3 batches of Fraxini Cortex（F. chinensis） dispensing granules were all>0.9， and 7 common peaks were identified. The results of cluster analysis and PCA showed that there was some differences in the composition of different batches of standard decoctions， but did not show aggregation of origin. As the standard decoctions， the extract rate was 6.18%-11.62%， the contents of esculin， syringin， fraxin， esculetin， fraxetin， calceolarioside B were 44.92-103.51， 1.36-11.87， 33.26-90.73， 4.63-29.75， 2.40-16.86， 2.49-17.35 mg·g-1， and the transfer rates from decoction pieces to standard decoction were 25.21%-42.54%， 52.57%-88.84%， 43.43%-79.45%， 49.15%-88.27%， 49.22%-72.69%， 27.66%-47.67%， respectively. The extract rates of Fraxini Cortex（F. chinensis） dispensing granules were 10.4%-10.7%， the transfer rates of the above six components from decoction pieces to dispensing granules were 42.76%-43.17%， 80.01%-80.90%， 59.59%-59.88%， 51.35%-52.67%， 60.50%-60.93%， 37.98%-38.37%， respectively， which were generally consistent with the transfer rates from decoction pieces to standard decoctions. ConclusionThe established quality control standard of Fraxini Cortex（F. chinensis） dispensing granules based on standard decoctions is reasonable and reliable， which can provide reference for the quality control and process research of this dispensing granules.
