Effect and Mechanism of Nongsuo Dangguiwan in Relieving Oxidative Stress in Premature Ovarian Insufficiency Rats Based on Nrf2/HO-1 Signaling Pathway
10.13422/j.cnki.syfjx.20230403
- VernacularTitle:基于Nrf2/HO-1信号通路探讨浓缩当归丸对缓解卵巢功能不全大鼠卵巢氧化应激损伤的影响
- Author:
Jiaqi LUO
1
;
Xiaoli DU
2
;
Dongmei CHEN
3
;
Miao CHEN
1
;
Na HU
2
;
Shasha XING
4
;
Wenzhao LIU
1
;
Ruiying TIAN
4
;
Li YANG
5
;
Jing WANG
1
;
Rui HE
4
;
Huiming MA
4
Author Information
1. Ningxia Medical University，Yinchuan 750004，China
2. Key Laboratory of Ningxia Minority Medicine Modernization Ministry of Education，Ningxia Medical University，Yinchuan 750004，China
3. Ningxia Key Laboratory of Stem Cell and Regenerative Medicine，General Hospital of Ningxia Medical University，Yinchuan 750004，China
4. Key Laboratory of Fertility Presevation and Maintenance（NXMU），Ministry of Education， Ningxia Medical University，Yinchuan 750004，China
5. Laboratory Animal Centre，Ningxia Medical University，Yinchuan 750004，China
- Publication Type:Journal Article
- Keywords:
Nongsuo Dangguiwan;
premature ovarian insufficiency;
sex hormones;
reactive oxygen species
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2023;29(13):1-9
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effect and mechanism of Nongsuo Dangguiwan in improving ovarian oxidative stress in rats with ovarian dysfunction. MethodThirty-six adult female SD rats were randomly divided into normal group， model group， positive drug group （Femoston， 0.3 mg·kg-1）， and high-， medium-， and low-dose groups of concentrated Nongsuo Dangguiwan （2.08， 4.16， 8.32 g·kg-1）， with six rats in each group. Rats， except for those in the normal group， were injected with 80 mg·kg-1 vinyl cyclohexene dioxide （VCD） per day for 14 consecutive days to induce ovarian dysfunction. From the 15th day， rats were treated with corresponding drugs by gavage， while those in the model group received 2 mL·kg-1 saline， once daily for 28 consecutive days. The ovarian index， levels of related hormones including estradiol （E2）， follicle-stimulating hormone （FSH）， luteinizing hormone （LH）， and anti-mullerian hormone （AMH） in serum， as well as superoxide dismutase （SOD） activity and glutathione （GSH） content in serum were measured by enzyme-linked immunosorbent assay （ELISA）. The malondialdehyde （MDA） content in serum was detected by the thiobarbituric acid （TAB） method. Ovarian morphology was observed by hematoxylin-eosin （HE） staining. The expression of nuclear factor E2-related factor 2 （Nrf2）， heme oxygenase-1 （HO-1）， SOD2， and SOD1 in ovarian tissues was detected by immunohistochemistry （IHC） and Western blot. ResultCompared with the normal group， the model group showed a significant reduction in growing follicles in the ovary， loose arrangement of granulosa cells in the follicle， decreased body weight， ovarian index， and serum AMH and E2 levels， increased LH and FSH levels （P<0.01）， reduced levels of SOD and GSH in serum （P<0.01）， and increased MDA level （P<0.01）. Compared with the model group， the groups with drug intervention showed increased ovarian index （P<0.05， P<0.01）， increased serum E2 level （P<0.05， P<0.01）， decreased FSH， AMH， and LH levels （P<0.05， P<0.01）， increased number of growing follicles in the ovary， potentiated SOD activity in serum， increased GSH content， decreased MDA content （P<0.05， P<0.01）， and up-regulated expression levels of Nrf2， HO-1， SOD2， and SOD1 proteins in ovarian tissues （P<0.05， P<0.01）. ConclusionNongsuo Dangguiwan can regulate serum hormone levels， increase the expression of Nrf2， HO-1， SOD2， and SOD1 in ovarian tissues， and improve ovarian antioxidant capacity to resist oxidative stress injury， thereby improving ovarian reserve function.
