Preliminary study on the mechanism underlying the ecological isolation of Oncomelania hupensis populations in Changde City
10.16250/j.32.1374.2022276
- VernacularTitle:常德市钉螺种群生态隔离机制的初步探析
- Author:
Shen CHEN
1
,
2
;
Lei DUAN
1
,
2
;
Shengming LI
3
;
Jie ZHOU
3
;
Yingcai ZHOU
4
;
Yuanzhi YANG
5
;
Mengli LIU
6
;
Yanren WANG
7
;
Shang XIA
1
,
2
,
8
;
Jing XU
1
,
2
;
Shan LÜ
1
,
2
,
8
Author Information
1. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), Key Laboratory of National Health Commission on Parasite and Vector Biology, WHO Collaborating Centre for Tropical Diseases
2. National Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai 200025, China
3. Hunan Institute of Schistosomiasis Control, China
4. Changde Center for Disease Control and Prevention, Hunan Province, China
5. Health Bureau of Taoyuan County, Changde City, Hunan Province, China
6. Health Bureau of Hanshou County, Hunan Province, China
7. Health Department of Dingcheng District, Changde City, Hunan Province, China
8. School of Global Health, Chinese Center for Tropical Diseases Research, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
- Publication Type:Journal Article
- Keywords:
Oncomelania hupensis;
Population genetics;
Ecological isolation;
Cytochrome C oxidase 1 gene;
Yuanjiang River;
Changde City
- From:
Chinese Journal of Schistosomiasis Control
2023;35(2):147-154
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate ecological isolation between Oncomelania hupensis snail populations in hilly regions and marshland and lake regions in Yuanjiang valley, Changde City, Hunan Province, and to unravel its underlying mechanisms. Methods Taoyuan County, Shimen County, Linli County and Lixian County in Changde City were selected as snail sampling sites in hilly regions, and Lixian County, Jinshi City, West Lake Administration District, Hanshou County and Dingcheng District were selected as snail sampling sites in marshland and lake areas. Cytochrome C oxidase 1 (cox 1) gene was amplified in snail samples and sequenced. The genetic sequences of O. hupensis snails were aligned using the software MEGA 11, and the haplotypes of O. hupensis snails were determined using the software DNASP 5.10.01. The phylogenetic tree was generated using Bayesian inference with the software MrBayes 3.2, and analysis of molecular variance (AMOVA) was performed to analyze the source of genetic divergence and estimate the genetic divergence index (FST) among snail populations with the software Arlequin 3.5.2.2. The genetic barrier among 11 O. hupensis snail populations was estimated using the Monmonier algorithm of adegenet toolkit in R package. The settings with “land in winter and water in summer” in the Yuanjian River section were divided into two categories according to the upstream and downstream, and the areas with “land in winter and water in summer” in the upstream and downstream were transformed into raster data, and then loaded into the software Fragstats 4 for analysis of landscape indicators. The trends in changes of digital elevation were extracted from the Yuanjiang River section based on the digital elevation model, and made three-dimensional visualization using the R package. Results The mitochondrial cox 1 gene were amplified in 165 O. hupensis snais from 11 sampling sites and sequenced, and a total of 152 valid gene sequences were obtained, with 46 haplotypes or 9 populations determined. No haplotype was shared in snails between Taoyuan County and Dingcheng District and Hanshou County along the downstream of the Yuanjiang River. The total area of settings with “land in winter and water in summer” was 617.66 hm2 in the upsteram of the Yuanjiang River, which consisted of 473 patches, with each patch measuring 1.31 hm2, the largest area index of 0.735 2, the landscape division index of 0.999 9, and the landscape shape index of 45.293 7. The total area of settings with “land in winter and water in summer” was 9 956.92 hm2 in the downstream of the Yuanjiang River, which consisted of 771 patches, with each patch measuring 12.91 hm2, the largest area index of 97.839 9, the landscape division index of 0.042 7, and the landscape shape index of 7.249 6. The area of settings with “land in winter and water in summer” was much larger in the downstream than that in the upstream of the Yuanjiang River, and the stronger landscape connectivity and non-remarkable alteration of riverbed elevation provided suitable habitats for snail breeding. Conclusion The hydrological and environmental characteristics of the upstream of the Yuanjiang River restrain the breeding and spread of O. hupensis, resulting in ecological isolation between Oncomelania hupensis in Taoyuan County and those in the downstream of Yuanjiang River.
