Mass spectrometry-guided study on cyclic peptides from sponge <i>Reniochalina</i> sp.

Zongmei WU; Ying WU; Shuaishuai Zhang; Can Kong; Shuping Wang; Houwen Lin

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Mass spectrometry-guided study on cyclic peptides from sponge Reniochalina sp.

VernacularTitle:质谱导向的肾指海绵Reniochalina sp.中环肽类成分研究
Author: Zongmei WU ¹ ; Ying WU ¹ ; Shuaishuai ZHANG ² ; Can KONG ³ ; Shuping WANG ¹ ; Houwen LIN ¹
Author Information

1. Department of Pharmacy, Renji Hospital Affiliated to School of Medicine, Shanghai Jiaotong University, Shanghai 200127, China.
2. School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China.
3. Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250103, China.
Keywords: Reniochalina sp.; chemical constituent; cyclic peptide; structure identification
From: Journal of Pharmaceutical Practice 2023;41(5):316-320
CountryChina
Language:Chinese
Abstract: Objective To study the cyclic peptides from sponge Reniochalina sp. under the guidance of mass spectrometry. Methods Mass spectrometry-guided procedural separation methods were used to track and isolate the cyclic peptides from the sponge genus Reniochalina. The structures of compounds were elucidated by the determination of physicochemical parameters and comparison of spectroscopic data. The preliminary cytotoxic activity of compounds was assessed by the Cell Counting Kit-8 (CCK-8) method. Results Three cyclic peptides were isolated from the sponge Reniochalina sp. and identified as stylopeptide 1 (1), hymenamide D (2) and axinastatin 2 (3). Compound 1 exhibited cytotoxicity against six human cancer cell lines with IC₅₀ values ranging from 6.09 to 17.26 μmol/L. Conclusion Compound 1 - 3 were isolated from Reniochalina sp. for the first time, and compound 1 was a cytotoxic cyclic heptapeptide.