Effects of Huzhang Qingmai decoction on cognitive function and intestinal flora in mice with chronic cerebral ischemia
10.12206/j.issn.2097-2024.202208106
- VernacularTitle:虎杖清脉饮对慢性脑缺血小鼠认知功能及肠道菌群的影响
- Author:
Yuchen GUO
1
,
2
;
Huamin DING
2
;
Shanshan DENG
3
;
Yuefan ZHANG
3
;
Tiejun LI
1
,
2
Author Information
1. School of Pharmacy, Anhui University of Traditional Chinese Medicine, Hefei 230012, China
2. Punan Hospital of Pudong New Area, Shanghai 200125, China.
3. School of medicine, Shanghai University, Shanghai 200444, China.
- Keywords:
HZQMY;
chronic cerebral ischemia;
cognitive function;
16S rRNA;
intestinal flora
- From:
Journal of Pharmaceutical Practice
2023;41(5):302-309
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of action of Huzhang Qingmai decoction (HZQMY) on the improvement of cognitive function in mice with chronic cerebral ischemia from the perspective of intestinal flora. Methods A mouse model of chronic cerebral ischemia was established by placing microcoils around the bilateral common carotid arteries to induce bilateral carotid artery stenosis (BCAS). After 12 weeks of intragastric administration, the cognitive function of the mice was measured by the Morris water maze; the myelin damage was analyzed by LFB staining; The contents of the cecum of the mice in each group were extracted and analyzed by 16S rRNA sequencing. Results The results of the water maze experiment showed that the mice in the HZQMY group had a significantly shorter escape latency, increased the number of crossings platform and the percentage of target quadrants. LFB staining showed that the white matter damage in the model group was severe; the white matter damage in the HZQMY group was milder. The results of 16S rRNA sequencing showed that compared with the model group, the abundance of Verrucomicrobiota, Akkermansia, and ErysiPelatoclostridium capsulatum in the intestinal flora in HZQMY group was significantly reduced (P<0.05), while the abundances of Eubacterium_xylanoPhilum and Allobaculum were significantly increased (P<0.05). Conclusion The protective effect of HZQMY, which has the effect of improving cognitive function in mice with chronic cerebral ischemia, may be related to the regulation of intestinal flora in mice with chronic cerebral ischemia.
