Construction and in vitro evaluation of an LNP system for mRNA delivery
10.12206/j.issn.2097-2024.202302026
- VernacularTitle:mRNA脂质纳米粒载药系统的构建及体外评价
- Author:
Xinlu CHEN
1
;
Yuan GAO
2
;
Juanjuan LI
1
;
Huanhuan GUO
1
;
Zhuo WANG
1
;
Shen GAO
1
Author Information
1. The First Affiliated Hospital, Naval Medical University, Shanghai 200433, China.
2. School of Pharmacy, Fudan University, Shanghai 200032, China.
- Keywords:
LNP;
DLin-MC3-DMA;
mRNA;
drug delivery
- From:
Journal of Pharmaceutical Practice
2023;41(5):291-295
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct lipid nanoparticles DLin-LNP for mRNA delivery. Methods DLin-LNP was prepared by thin film hydration method, and DLin-LNP/mRNA was further constructed by using EGFP-mRNA as model drug. The particle size, zeta potential, and appearance morphology were measured. Furthermore, the intracellular distribution and transfection of DLin-LNP/mRNA in RM-1 cells was investigated by laser scanning confocal microscope. Results DLin-LNP was successfully prepared. The average particle size was about (151.1±2.1) nm, the no-load potential was (23.7±0.5) mV. The cytotoxicity of DLin-LNP was far lower than that of the commercially available liposomal Lipo8000. The results of transfection experiment indicated that DLin-LNP has high transfection efficiency for mRNA delivery with low cytotoxicity and good stability. Conclusion DLin-LNP could become a potential mRNA vector for gene therapy.