MTT and Neutral red Biologic Assay for Human Bladder Cancer Cell Line(TCC-SuP) and Androgen Stimulated Cell Line of Mouse (SC-115): In Vitro Chemosensitivity Test.
- Author:
Bup Wan KIM
1
;
Duk Youn KIM
;
Sung Kwang CHUNG
;
Sae Kook CHANG
Author Information
1. Department of Urology, School of Medicine, Kyungpook National University, Taegu, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
MTT;
Neutral red;
TCC-SuP;
SC-115
- MeSH:
Animals;
Antineoplastic Agents;
Artifacts;
Biological Assay*;
Cell Line*;
Cisplatin;
Doxorubicin;
Humans*;
Methotrexate;
Mice*;
Neutral Red*;
Staining and Labeling;
Urinary Bladder Neoplasms*;
Urinary Bladder*
- From:Korean Journal of Urology
1990;31(5):635-640
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
To overcome the limitations of clonogenic assay (low plating efficiencies, clumping artifacts, long assay duration), we performed chemosensitivity test using MTT and neutral red biologic assay on SC-115 (androgen stimulated mouse tumor) and TCC-SuP (human bladder tumor) cell lines. The anticancer agents tested were adriamycin, cisplatin, methotrexate and combination of these drugs. And the following results were obtained ; 1) The growth of two cell lines were inhibited dose-dependently by application of adriamycin, cisplatin and combination of drugs. But methotrexate was not effective on TCC-SuP cell line. 2) The neutral red biologic assay is more convenient and rapid method than MTT assay but staining ability is superior in MTT assay. 3) The MTT and neutral red biologic assay offer advantages in speed, simplicity, cost & safety for the chemosensitivity test and for determining the new regimen.
