High-throughput screening of differential expression of exosomal miRNAs in DeBakey typeⅠacute aortic dissection patients
- VernacularTitle:高通量筛选DeBakeyⅠ型急性主动脉夹层患者外泌体miRNAs差异表达
- Author:
Dan ZHANG
1
;
Xiang ZHAO
1
;
Xiaoyao LIU
1
;
Aizezi AYITILA
1
;
Xiang MA
1
Author Information
1. The Second Department of Coronary Heart Disease, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, 830054, P. R. China
- Publication Type:Journal Article
- Keywords:
Acute aortic dissection;
exosomes;
miRNAs;
bioinformatic analysis
- From:
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
2023;30(02):253-259
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the changes in the expression and significance of serum exosomal miRNAs in patients with DeBakey typeⅠacute aortic dissection (AAD). Methods Twelve male patients with AAD and six healthy male medical examiners from our hospital were retrospectively included in this study. According to the time of chest pain, the AAD patients were divided into an AAD group within 24 h of chest pain onset, aged 47.00±8.79 years and an AAD group within 48 h of chest pain onset, aged 50.17±9.99 years. The healthy males were allocated to a control group, aged 49.17±4.26 years. Serum exosomal miRNAs were isolated, identified and quantified, and then differentially expressed exosomal miRNAs were screened. The bioinformatic analyses such as GO and KEGG were performed on the differentially expressed exosomal miRNAs. Results High-throughput screening results revealed differential expression of AAD serum exosomal miRNAs. The upregulated miRNAs of AAD groups was hsa-miR-574-5p (P<0.05), and downregulated miRNAs were hsa-miR-223-3p, hsa-miR-146b-5p, hsa-miR-15b-5p, and hsa-miR-155-5p (P<0.05). Further bioinformatic analysis of the above miRNAs revealed that they were mainly enriched in signaling pathways such as transforming growth factor-β, cell cycle and endoplasmic reticulum protein synthesis. Conclusion Differential expressions of serum exosomal miRNAs in AAD patients may be related to the pathogenesis of AAD, providing new ideas and clues for further exploration of AAD diagnostic markers and pathogenesis.
