Adaptive response of A549 cell apoptosis induced by low-dose X-ray irradiation
10.13491/j.issn.1004-714X.2022.02.001
- VernacularTitle:低剂量 X 线辐射对 A549 细胞凋亡的适应性反应
- Author:
Xiaoling YU
1
;
Li RONG
1
;
Fang FANG
1
;
Xiaowen DING
1
;
Jue LI
1
;
Xiaochun WANG
1
Author Information
1. Beijing Prevention and Treatment Hospital of Occupational Disease for Chemical Industry (Beijing Institute of Occupational Disease Prevention and Treatment)
- Publication Type:Journal Article
- Keywords:
Low-Dose irradiation;
X-Ray;
Adaptive effect;
Cell apoptosis;
Cell cycle
- From:
Chinese Journal of Radiological Health
2022;31(2):139-143
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the adaptive response and time effect of A549 cell apoptosis induced by low-dose X-ray
irradiation, and to preliminarily explore the possible mechanism of adaptive effect.
Methods:A549 cells were irradiated with X-ray of 50 mGy, 200 mGy and 500 mGy, respectively, and then irradiated with an effect dose of 20 Gy after intervals of 3 h, 6 h, 12 h, 24 h and 48 h, respectively, for cell apoptosis detection. The cell cycle distribution and DNA damage were detected after an interval of 6 h between the initial dose and the effect dose. 20 Gy and 0 Gy were set as the control.
Results:After irradiation at 20 Gy at intervals of 3 h, 6 h, 12 h and 24 h from the low- dose irradiation, the apoptosis rates of the 50 mGy～20 Gy, 200 mGy～20 Gy, and 500 mGy~ 20 Gy groups were significantly lower than that of the 20 Gy group
(P < 0.05); after an interval of 48 h, there was no significant difference in the apoptosis rate between the 50 mGy～20 Gy, 200 mGy～20 Gy, and 500 mGy～20 Gy groups and the 20 Gy group. After an interval of 6 h between the low-dose irradiation and the effect dose irradiation, the percentage of cells at G0/G1 phase in the 50 mGy～20 Gy and 200 mGy～20 Gy groups was significantly lower than that in the 20 Gy group (P < 0.05); the percentage of cells at G2/M phase in the 50 mGy～20 Gy and 200 mGy~20 Gy groups were significantly reduced compared with the 20 Gy group (P < 0.05). There was no significant difference in the percentage of cells at G0/G1 and G2/M phases between the 500 mGy～20 Gy and 20 Gy groups. Compared with the 20 Gy group, the cell DNA damage in the 50 mGy～20 Gy, 200 mGy～20 Gy and 500 mGy~20 Gy groups were decreased, but without significant difference.
Conclusion:Low-dose X-ray irradiation can induce the adaptive response of A549 cells apoptosis, which is related to the time interval between the initial dose and the effect dose. The adaptive effect may be related to the changes in cell cycle induced by low-dose X-ray.
