Determination of six arsenic species in human urine by HPLC-ICP-MS
10.19428/j.cnki.sjpm.2023.22316
- VernacularTitle:高效液相色谱-电感耦合等离子体质谱法测定人尿中6种砷形态化合物
- Author:
Yi HE
1
;
Lanxia LIU
2
;
Liming XUE
1
;
Libei XIONG
1
;
Yamin FANG
1
Author Information
1. Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, China
2. Xuhui Center for Disease Control and Prevention, Shanghai 200237, China
- Publication Type:Journal Article
- Keywords:
HPLC;
ICP mass spectrometry;
arsenical;
human being;
urine
- From:
Shanghai Journal of Preventive Medicine
2023;35(2):182-186
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo establish an High Performance Liquid Chromatography-Inductively Coupled Plasma-Mass Spectrometry （HPLC-ICP-MS） method for determination of six arsenic species in human urine，including arseniccholine （AsC）， arsenobetaine （AsB）， arsenite （As3+）， dimethylarsinic acid （DMA5+）， monomethylarsonic acid （MMA5+）， and arsenate （As5+）. MethodsThe pH value of mobile phase and the content of anhydrous ethanol were optimized. Ammonium carbonate （50 mmol·L-1， containing 2% anhydrous ethanol， pH-8.5） mobile phase was selected. Cl- interference was eliminated by He mode. The arsenic species in 10-fold diluted human urine samples were separated by an Hamilton PRP X-100 anionic column. A method for the determination of six arsenic species was established. ResultsSix arsenic species could be separated in 13 minutes. The linear correlation coefficients were above 0.999. The limits of detection were 0.10‒0.20 μg·L-1， and the limits of quantification were 0.30‒0.50 μg·L-1. Precision experiments showed that RSD ranged from 5.96% to 9.07% when adding concentration 0.20 μg·L-1； from 2.48% to 6.38% when adding concentration 2.00 μg·L-1； and from 1.41% to 2.57% when adding concentration 5.00 μg·L-1. Accuracy test showed that the recoveries were 80%‒125%. ConclusionThe established HPLC-ICP-MS method for determination of six arsenic species in human urine is rapid， accurate and sensitive. It can be applied to the determination of arsenic species in human urine.
