Detection result of virulence factors in Staphylococcus aureus isolated from retail beef
- VernacularTitle:Үхрийн түүхий махнаас ялгасан Staphylococcus aureus-ийн хоруу чанарыг тодорхойлсон дүн
- Author:
Anujin B
1
;
Munkhdelger Ya
2
;
Urangoo Z
3
;
Udval U
2
;
Sarantuya J
2
Author Information
1. Ach Medical University
2. School of Biomedicine, Mongolian National University of Medical Sciences
3. National Cancer Center of Mongolia
- Publication Type:Journal Article
- Keywords:
antimicrobial resistance;
foodborne disease;
retail beef;
Staphylococcus aureus
- From:Mongolian Medical Sciences
2020;191(1):3-7
- CountryMongolia
- Language:Mongolian
-
Abstract:
Introduction:In the United States, Staphylococcus aureus (S.aureus) is considered one of the top five pathogens
causing domestically acquired foodborne diseases and is responsible for an estimate of 241,000
illnesses per year. Foods that have been frequently implicated in Staphylococcal food-borne disease
are meat, meat products, egg products, milk, dairy products, salads and bakery products. β-lactam
antibiotics are routinely prescribed for treating S. aureus caused infections, but antibiotic resistance
is increasing at an alarming rate.
Aim:Detection of virulence factors and antibiotic resistance in S.aureus isolated from retail beef
Materials and Methods :A total of 100 meat samples were collected from markets including Kharkhorin 28, Bars 4, Bayanzurkh
15, Huchit shonkhor 33, Denjiin myanga 4 and Bumbugur 16. S.aureus strains were determined on
the basis of MNS 6308:2012 standard using Baird-Parker selective agar and confirmed by polymerase
chain reaction (PCR) in retail beefs. Bacterial susceptibility to antimicrobial agents determined by the
Kirby Bauer disk diffusion method.
Results:Overall, 81% meat samples were contaminated with staphylococcal of which 54.3% were low, 28%
were moderate, 11.1% were high and 6.1% were very high. PCR amplification of the thermostable
nuclease-encoding nuc gene using the gene-specific primers and the chromosomal DNA preparation
yielded a 270 bp amplicon, as expected and 35 (43.2%) confirmed as S. aureus. According to the
findings of the current study, S.aureus strains isolated from the beef were high resistant (88.6%
-97.1%) to antibiotics of penicillins group and low resistant (8.6%) to chloramphenicol. In total, 48.6%
of isolates were multidrug resistant.
Conclusion:The contamination of staphylococcal was high in retail beef in Ulaanbaatar. Most S.aureus isolates
exhibited resistance to a antibiotics of penicillin group. The half of the isolates were multidrug resistant
and high virulence.
- Full text:2020-191(1)-3-7.pdf
