Effect of Immunos on immune response cells by azathioprine Induced immunosuppression in mice
- VernacularTitle:Хулганад азатиопринээр үүсгэсэн дархлаа дутагдлын үед дархлааны эсүүдийн хариу урвалд Иммунос бэлдмэлийн үзүүлэх нөлөөг судалсан дүн
- Author:
Davaasambuu T
1
;
Oyunchimeg B
1
;
Bayanmunkh A
1
;
Sosorburam B
1
;
Odchimeg B
1
;
Badamtsetseg B
1
;
Lkhagva L
1
;
Khurelbaatar L
2
Author Information
1. Drug Research Institute
2. Monos Group
- Publication Type:Journal Article
- Keywords:
Azathioprine;
Cytokine CD4;
Cytokine CD8;
Immunoglobulin;
Immunostimulator, Splenocyte;
White Blood Cells
- From:Mongolian Medical Sciences
2018;186(4):47-51
- CountryMongolia
- Language:Mongolian
-
Abstract:
Background:Herbal medicines continue to be widely used as natural promoters of good health, as immune-modulators
in recent years. This situation is directly related to the rapid growth of natural based products, the decrease of chemical synthesized products and as well as the increase of natural substance consumption.
Objective:The purpose of this survey was to study influence of Immunos herbal medicines on immune system in the
experimental and preclinical circumstances.
Materials and Methods:The immune deficiency was to created by Azathioprine through 5 days in the white mice after that control
group, preparation of Immunal, Salimon and Immunos 1, 2 were administrated appropriate doses by oral
during 10 days. Then we collected blood and quantified number of white blood cells (K/µL), quantity of
splenocyte (×106 cell/ml), amount of CD4+, CD8+ and IgM, IgA, Ig G (mg/ml) (Elisa Kit Assay: Catalog.
No: WAM-568 (Elisa Reader, 450 nm)-WKEA MED SUPPLIES CORP) on the 5th, 10th days.
Results:All statistical analyses were conducted with SPSS version 20.0 software (IBM, Armonk, NY). One-way
ANOVA was used to assess statistical significance between Immunos groups and days of observation.
Mean values of white blood cells in blood, quantity of splenocyte, CD4+, CD8+ and IgM, IgG levels determined in the control and experimental groups. White blood cells level were significantly increased in the Immunos group compared with the control group by 55.6 percent (11.5±0.9 K/µL vs 5.1±0.51 K/µL, p<0.001) and number of splenocyte increased Immunos group compared with the control group by 60.6
% (352.2±23.5 ×106 cell/ml vs 138.6±23.5 ×106 cell/ml, p<0.01). Therefore, CD4+, CD8+ and IgM, IgG
levels were significantly increased in the Immunos group compared with the control group by 0.71 to
8.8% (IgG: 11.47±0.42 vs 10.45±0.43 μg/ml, IgM: 11.33±0.81 vs 10.48±0.31 μg/ml, CD4+: 10.44±0635
vs 10.04±0.372 U/ml, CD8+: 9.75±1.02 vs 9.68±0.45 U/ml p<0.02).
Conclusion:It’s concluded that, Immunos preparation shows immune-stimulator effect in cellular immunity and humoral immunity in the case of immunosuppressant by Azathioprine.
- Full text:2018-186(4)-47-51.pdf
