mRNA Expression of Aquaporins in Human Luteinized Granulosa Cells from Different Diameter Follicles
10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.2023.0313
- VernacularTitle:人类水通道蛋白在不同径线卵泡的黄素化颗粒细胞中的表达水平
- Author:
Ze-tong ZHENG
1
;
Ji-fan TAN
1
;
Yu-bin LI
1
;
Xiu-bing ZHANG
1
;
Si-min LIU
1
;
Can-quan ZHOU
1
Author Information
1. Reproductive Center， The First Affiliated Hospital， Sun Yat-sen University//Guangdong Key Laboratory of Reproductive Medicine， Guangzhou 510080， China
- Publication Type:Journal Article
- Keywords:
aquaporins （AQPs）;
luteinized granulosa cells;
follicular fluid;
follicle growth
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2023;44(3):462-470
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the mRNA expression levels of various aquaporins （AQPs） in luteinized granulosa cells from follicles of different diameters. MethodsFrom March 25， 2022 to September 23， 2022 in our reproductive medicine center， 48 women undergoing in-vitro fertilization （IVF） were enrolled and divided into the antagonist group and the agonist group according to the ovarian stimulation protocol. Follicular fluid samples were collected on the day of oocyte pick-up and granulosa cells were extracted from follicles of different diameters： small （<13 mm）， medium （13~18 mm） and large （≥18 mm）. After RNA quantification， 22 cases （66 samples） were included for analysis and mRNA expression levels of AQPs were compared among the three follicle groups. ResultsThe mRNA expression of aquaporin 2 （AQP2） in luteinized granulosa cells increased with the increase of follicle diameter （linear trend P = 0.004） and the difference was statistically significant between two groups of large and small follicles （P = 0.017）. Statistical difference was found in the antagonist group （P = 0.049 6）， but not in the agonist group （P = 0.108）. ConclusionThe mRNA level of AQP2 in luteinized granulosa cells increases with the increase of follicle diameter and its expression is related to the ovarian stimulation protocol， suggesting that AQP2 may play a role in follicle growth and follicular fluid formation， and its mRNA expression level may be regulated by follicle stimulating hormone （FSH） and luteinizing hormone （LH）.
