Scutellaria Baicalensis Polysaccharides Alleviates the Inflammation of DSS-induced UC Model Mice by Regulating JAK2/STAT3 Pathway and IL-23/IL-17 Inflammatory Axis
10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.2023.0308
- VernacularTitle:黄芩多糖通过调节JAK2/STAT3通路和IL-23/IL-17炎性轴改善DSS诱导的UC模型小鼠的炎症
- Author:
Jie MA
1
;
Yun-zhi CHEN
2
;
Lei TIAN
1
Author Information
1. Department of Gastroenterology， The First Affiliated Hospital of Jinzhou Medical University， Jinzhou 121000， China
2. Department of Gastroenterology， Huludao Central Hospital， Huludao 125000， China
- Publication Type:Journal Article
- Keywords:
Scutellaria Baicalensis polysaccharide;
ulcerative colitis;
IL-17;
IL-23;
JAK2/STAT3 pathway
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2023;44(3):423-429
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effect and mechanism of Scutellaria Baicalensis polysaccharides on intestinal immunity in mice with ulcerative colitis （UC）. MethodsC57BL/6 mice were randomly assigned to blank group， model group， mesalazine group and Scutellaria Baicalensis polysaccharides low-dose， medium-dose and high-dose groups （n=10 mice/group）. The daily status of mice was observed and the disease activity index （DAI） score was recorded. The expression of cytokines including IL-6， IL-17 and IL-23 in serum was detected by ELISA. After the mice were sacrificed， HE was used to observe the intestinal mucosal damage in mice， and the colonic tissue damage index （TDI） score was recorded. Western blot and immunohistochemistry were used to detect the expression levels of Janus kinase 2 （JAK2）， signal transducer and activator of transcription （STAT3）， p-JAK2 and p-STAT3 proteins. ResultsCompared with the blank group， the DAI score of the model group was significantly increased， the contents of IL-6， IL-17 and IL-23 in serum were significantly increased， the ratio of p-JAK2/JAK2 and p-STAT3/STAT3 in colon tissue was significantly increased， and the expression levels of p-JAK2 and p-STAT3 were significantly increased. Compared with the model group， the DAI score of mice in each administration group was significantly decreased. The contents of IL-6， IL-17 and IL-23 in serum were decreased， the TDI score was decreased， the ratio of p-JAK2/JAK2 and p-STAT3/STAT3 in colon tissue was decreased， and the expression of p-JAK2 and p-STAT3 was decreased. ConclusionScutellaria Baicalensis polysaccharides may improve the inflammatory effect of UC mice through JAK2/STAT3 pathway and IL-23/IL-17 inflammatory axis.
