Preparation and Biodistribution of 131I-labeled Hepatoma Nucleic Acid Nanotrain
10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.2023.0307
- VernacularTitle:131I标记的肝癌核酸纳米火车的制备及生物分布研究
- Author:
Xue LIN
1
;
Jie-hua XU
2
;
Ya-ni DUAN
1
;
Yan-qiu ZHU
1
;
Yuan-yuan CHU
1
;
Ming-ming LIU
1
;
Jie QIN
1
Author Information
1. Department of Radiology， The Third Affiliated Hospital of Sun Yat-sen University， Guangzhou 510630， China
2. Department of Nuclear Medicine， Zhuhai People's Hospital， Zhuhai 519000， China
- Publication Type:Journal Article
- Keywords:
hepatocellular carcinoma;
aptamer;
nanotrain;
radioiodinated labeling;
biodistribution
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2023;44(3):416-422
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo construct 131Ⅰ-labeled hepatoma nucleic acid nanotrain and to explore its feasibility as a new nuclide carrier targeting hepatoma. MethodsThree short nucleic acid chains self-assembled to a long nucleic acid chain after being annealed， and 131Ⅰ-NT was obtained by radioiodine labeling using chloramine T method. The labeling efficiency and radiochemical purity of the nanoparticles were measured by paper chromatography. The stability of the labeled products in vitro at different temperatures and different storage solvents was detected. The specific uptake of nanoparticles by hepatocellular carcinoma cells was observed by laser confocal microscopy， and the radioactive uptake ratio of 131Ⅰ-NT combined with human hepatocellular carcinoma cell HepG2 and normal hepatocyte L02 was measured. The biodistribution of 131Ⅰ-NT was obtained through injecting 131Ⅰ-NT into HepG2 tumor-bearing mice via tail vein. ResultsThe labeling rate of 131Ⅰ-NT was （93.05±0.74） %， and the radiochemical purity post purification was （98.35±0.32） %. Its radiochemical purity in PBS and pure serum at 4℃ for 24 h was （92.77±0.04） % and （89.43±0.2） %， respectively. The radioactivity uptake rate of HepG2 cells was higher than that of L02 cells after 131Ⅰ-NT was incubated with two kinds of cells for 2 h significantly. After injection of 131Ⅰ-NT through tail vein， the radioactive uptake per gram of tumor tissue were （4.9±0.55）%ID/g， （10.12±0.32）%ID/g and （4.25±0.31）%ID/g at 30 min， 1 h and 2 h， respectively. The T/M ratio was 7.33±2.04， 36.54±12.72 and 44.93±7.90 respectively. ConclusionsThe 131Ⅰ-labeled long chain nucleic acid nanotrain was constructed successfully， which possesses relatively high stability in vitro ， and high targeting ability to HepG2 cells in vitro and HepG2 tumor-bearing mouse model. Our study demonstrated that 131Ⅰ-NT may be a potential radionuclide carrier targeting human liver cancer， which provides a new idea for the targeted radionuclide diagnosis and treatment of hepatocellular carcinoma.
