Akkermansia Muciniphila Alleviates Visceral Hypersensitivity in Irritable Bowel Syndrome Rats via Regulating CB2R
10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.20230418.001
- VernacularTitle:嗜粘蛋白阿克曼氏菌通过调控CB2R缓解肠易激综合征大鼠内脏高敏
- Author:
Lin XIAO
1
;
Qin LIU
1
;
Li-shou XIONG
1
Author Information
1. Department of Gastroenterology and Hepatology， The First Affiliated Hospital of Sun Yat-sen University， Guangzhou 510080， China
- Publication Type:Journal Article
- Keywords:
Akkermansia muciniphila （A. muciniphila）;
cannabinoid receptor type 2 （CB2R）;
irritable bowel syndrome （IBS）;
visceral hypersensitivity
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2023;44(3):379-385
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the mechanism of Akkermansia muciniphila （A. muciniphila） regulating the visceral hypersensitivity in irritable bowel syndrome （IBS） rats induced by neonatal maternal separation （MS） and water avoidance stress （WAS）. MethodsNeonatal male Sprague-Dawley rats were randomly divided into sham WAS group （blank group）， MS+WAS group （IBS model group） and A. muciniphila group. IBS model was established by MS combined with WAS in both IBS model group and A. muciniphila group. Meanwhile， the rats in the A. muciniphila group were given 1 mL 1×109 CFU/mL A. muciniphila by gavage daily for 10 days. Visceral pain responses were detected by behavioral observations and abdominal withdrawal reflex scores. ResultsCompared with IBS model group， A. muciniphila group exhibited significant increase of body weight and visceral pain threshold， significantly decreased numbers of fecal particles and proportions of unformed stools， significantly higher expression levels of cannabinoid receptor type 2 （CB2R） mRNA in colon tissues. ConclusionA. muciniphila may alleviate the visceral hypersensitivity in IBS rats by regulating the expression of CB2R mRNA in colonic tissues.
