Inhibitory action of Lipopolysaccharide-induced signal transductions by Valproic acid

Ulziisaikhan J; Tsogtsaikhan S; Yokochi T; Enkhsaikhan L; Jambaldorj J; Javkhlan B; Baigalmaa B; Tsevelmaa N; Galindev B; Sodnomtsogt L; Munkhtuvshin N; Munkhbat B; Bilegtsaikhan Ts

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Inhibitory action of Lipopolysaccharide-induced signal transductions by Valproic acid

VernacularTitle:Липополисахаридаар өдөөгдөх дархлааны хариу урвалд вальпроатын хүчлийн нөлөөг судлах нь
Author: Ulziisaikhan J ¹ ; Tsogtsaikhan S ¹ ; Yokochi T ² ; Enkhsaikhan L ¹ ; Jambaldorj J ^{3
,

4} ; Javkhlan B ^{1
,

5} ; Baigalmaa B ⁵ ; Tsevelmaa N ⁵ ; Galindev B ⁵ ; Sodnomtsogt L ⁵ ; Munkhtuvshin N ⁴ ; Munkhbat B ⁵ ; Bilegtsaikhan Ts ^{4
,

5}
Author Information

1. Department of Microbiology and Immunology, School of Biomedical Sciences, MNUMS
2. Department of Microbiology and Immunology, Aichi Medical University School of Medicine
3. Department of Molecular Biology and Genetics, School of Biomedical Sciences, MNUMS
4. Central Scientific Research Laboratory, IMS
5. Core Laboratory, Science and Technology Center, MNUMS
Publication Type:Journal Article
Keywords: Valproic acid; Lipopolysaccharide; PI3K; Akt
From: Health Laboratory 2019;9(1):12-20
CountryMongolia
Language:Mongolian
Abstract: Introduction:Valproic acid (VPA) has been used in the treatment of seizures and bipolar disorders. In the present study, we examined how VPA affected PI3K-Akt pathway in response to LPS by using mouse RAW 264.7 macrophage cells.
Material and methods:Mouse RAW 264.7 macrophage-like cells cultured and the cell viability checked by MTT and TUNEL assay. In addition, protein expression and protein interaction were detected by immune blotting and immune precipitation, respectively. TLR4 expression on cell surface studied by FACS analysis.
Results:The MTT and TUNEL assays demonstrated no significant difference between VPA at 2 mM treated and untreated control cells. VPA attenuated LPS-induced phosphorylation of phosphatidylinositol 3-kinase (PI3K) and Akt, but not nuclear factor (NF)-κB and mitogen activated protein kinases (MAPKs). There was no significant difference in the TLR4 expression on the cell surface between cells treated with or without VPA. VPA inhibited LPS-induced PI3K/Akt signal transduction in a dose dependent manner.
Conclusion:VPA at 2mM exhibits nontoxic effect in the RAW 264.7 cells. VPA down regulates LPS-induced phosphorylation of Akt via inhibition of PI3K activation.
Full text:HL-2019-9(1)-12-20.pdf