Effects of low-dose radiation on oxidative stress and damage repair in HBE cells
10.13491/j.issn.1004-714X.2023.02.012
- VernacularTitle:低剂量电离辐射对HBE细胞氧化应激及损伤修复影响
- Author:
Linqian ZHOU
1
,
2
;
Weixu HUANG
2
;
Lina CAI
3
,
4
;
Weiyi KE
3
,
4
;
Lingyu ZHANG
1
,
2
;
Yashi CAI
3
,
5
;
Sufen ZHANG
2
;
Ping YANG
6
;
Jianming ZOU
2
;
Huifeng CHEN
1
,
2
Author Information
1. School of Public Health, Guangzhou Medical University, Guangzhou 511436 China
2. Guangdong Key Laboratory of Occupational Disease Prevention and Control, Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou 510300 China.
3. Guangdong Key Laboratory of Occupational Disease Prevention and Control, Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou 510300 China
4. School of Public Health, Guangdong Pharmaceutical University, Guangzhou 510006 China.
5. School of Public Health, Southern Medical University, Guangzhou 510515 China.
6. School of Public Health, Guangzhou Medical University, Guangzhou 511436 China.
- Publication Type:Journal Article
- Keywords:
Low-dose ionizing radiation;
Oxidative stress;
Oxidative DNA damage;
DNA repair
- From:
Chinese Journal of Radiological Health
2023;32(2):150-155
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of lowdose ionizing radiation (LDIR) on oxidative stress and damage repair in human bronchial epithelial (HBE) cells. Methods HBE cells were divided into 0, 50, 100, and 200 mGy groups, and cultured for 24 and 48 h after X-ray irradiation, respectively. The cell viability, levels of glutathione (GSH), malondialdehyde (MDA), and 8-hydroxy-2’-deoxyguanosine (8-OHdG), and transcriptional levels of DNA damage repair genes PPP2R2D and TP53 were measured. Results At 24 h after irradiation, there was no significant difference in the cell viability between the dose groups and the control group (P > 0.05); all dose groups had significantly increased MDA level, dose-dependently decreased GSH level, dose-dependently increased 8-OHdG level, and significantly increased mRNA level of PPP2R2D gene (all P < 0.05); the mRNA expression level of TP53 gene was significantly increased in the 50 mGy group (P < 0.05). At 48 h after irradiation, there were the highest cell viability, significantly decreased MDA and 8-OHdG levels, and significantly increased mRNA expression levels of PPP2R2D and TP53 genes in the 50 mGy group compared with the control group (all P < 0.05); the GSH level in the 100 mGy group was significantly increased (P < 0.05). Conclusion LDIR, especially radiation at 50 mGy, can affect the oxidative-antioxidant level in HBE cells and the transcript-level differential expression of DNA damage repair genes.
