Effect of Loulianwan on Gut Microbiota in db/db Mice with Type 2 Diabetes Mellitus Based on 16S rRNA Sequencing Technology
10.13422/j.cnki.syfjx.20222107
- VernacularTitle:基于16S rRNA技术研究蒌连丸对2型糖尿病db/db小鼠肠道菌群的影响
- Author:
Chuan PENG
1
;
Xuefang HU
1
;
Zhengtao CHEN
1
;
Chenyu DOU
2
;
Peng YANG
2
;
Lei DING
3
;
Lili WU
4
;
Lingling QIN
5
;
Tonghua LIU
4
Author Information
1. School of Clinical Medicine，Chengdu University of Traditional Chinese Medicine，Chengdu 610036，China
2. The First School of Clinical Medicine，Shaanxi University of Chinese Medicine，Xi'an 711301，China
3. Second Clinical College，Beijing University of Chinese Medicine，Beijing 100078，China
4. Institute of Chinese Medicine Health Preservation，Beijing University of Chinese Medicine，Beijing 100029，China
5. Beijing University of Chinese Medicine，Beijing 100029，China
- Publication Type:Journal Article
- Keywords:
Loulianwan;
type 2 diabetes mellitus;
glycolipid metabolism;
16S rRNA sequencing technology;
db/db mice
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2023;29(12):63-70
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effect of Loulianwan on the gut microbiota of db/db mice with type 2 diabetes mellitus （T2DM）. MethodMale db/m+ mice aged 4-5 weeks were assigned to the normal group， and male db/db model mice of the same age were randomly divided into model group， metformin group （0.25 g·kg-1·d-1）， and Loulianwan group （13 g·kg-1·d-1）， with six mice in each group. Drug intervention lasted five weeks. The body weight， water intake， and fasting blood glucose （FBG） of the mice were recorded every week. After five weeks， the FBG， liver triglyceride （TG）， liver total cholesterol （TC）， glycated serum protein （GSP）， and fasting serum insulin （FINS） were detected， and the insulin resistance index （HOMA-IR） was calculated. The feces in the mouse intestines were collected， and the 16S rRNA sequencing technology was used to detect the structural changes in the fecal gut microbiota of mice in each group. ResultCompared with the normal group， the model group showed increased body weight， water intake， FBG， liver TG， liver TC， GSP， FINS， and HOMA-IR （P<0.01）. Compared with the model group， the Loulianwan group showed reduced water intake， FBG， liver TG， liver TC， GSP， FINS， and HOMA-IR （P<0.01）. The gut microbiota in the Loulian Lills group changed from phylum to genus level. The relative abundance of beneficial bacteria increased and the relative abundance of harmful bacteria decreased. Among them， the abundance of Akkermansia muciniphila， Blautia， Ruminococcus， and Parabacteroides increased （P<0.01）. ConclusionLoulianwan can significantly improve glucose and lipid metabolism in db/db mice with T2DM， and its mechanism may be related to the increase in the abundance of Akkermansia muciniphila， Blautia， Ruminococcus， and Parabacteroides in the intestine.
