Study on influence of the CpG DNA on activation of IFN-γ signaling  transduction regulatory proteins

Baljinnyam T; Khulan U; Erkhembayar Sh; Baasansuren E; Javkhlan B; Batkhishig M; Enkhsaikhan L; Ulziisaikhan J; Baigalmaa B; Galindev B; Tsevelmaa N; Khongorzul B; Sodnomtsogt L; Munkhbat B; Munkhtuvshin N; Bilegtsaikhan Ts

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Study on influence of the CpG DNA on activation of IFN-γ signaling transduction regulatory proteins

VernacularTitle:IFN-γ-ийн дохио дамжилтад оролцдог зохицуулагч уургийн идэвхжилд СpG ДНХ-ийн нөлөөг судалсан дүн
Author: Baljinnyam T ^{1
,

2} ; Khulan U ² ; Erkhembayar Sh ² ; Baasansuren E ² ; Javkhlan B ² ; Batkhishig M ² ; Enkhsaikhan L ² ; Ulziisaikhan J ² ; Baigalmaa B ² ; Galindev B ² ; Tsevelmaa N ² ; Khongorzul B ² ; Sodnomtsogt L ² ; Munkhbat B ² ; Munkhtuvshin N ¹ ; Bilegtsaikhan Ts ²
Author Information

1. Institute of Medical Sciences named after Shagdarsuren T.
2. Mongolian National University of Medical Sciences
Publication Type:Journal Article
Keywords: CPG ДНХ; IFN-γ; p38; PIAS1; SOCS1; SHP2
From:Mongolian Medical Sciences 2018;186(4):10-13
CountryMongolia
Language:Mongolian
Abstract: Introduction:When human body encounters external pathogens primary/innate immunity cells are activated by recognizing them and secondary/adaptive immunity is activated consecutively. In our previous study, we revealed that there is a synergistic action between TLR9 and IFN-γ signaling in the endothelial cells.
Purpose:To determine the role of negative and positive regulator proteins on the IFN-γ/TLR9 signaling pathway.
Methods:In this study, murine endothelial cell (END-D) culture was used. END-D cells pre-treated with TLR9 ligand CpG DNA and then stimulated with IFN-γ. The negative (SHP-2, SOCS1, PIAS1) and positive (p38) regulator protein expression was detected by Western blotting.
Results and Conclusion:Treatment by TLR9 ligand CpG DNA and IFN-γ increased positive regulator p38 phosphorylation in 0.5 hour. CpG DNA inhibited IFN-γ negative regulator PIAS1 protein expression in 6 hour and SOCS1 and SHP-2 expression could not affect in 4 hour.
Full text:2018-186(4)-10-13.pdf