Detection results of lead resistant bacteria from soil and water in Ulaanbaatar city
- VernacularTitle:Улаанбаатар хотын хөрс болон уснаас хар тугалгад тэсвэртэй бактери илрүүлэх судалгааны дүнгээс
- Author:
Munkhjin B
1
;
Altanchimeg N
1
;
Tumenjargal D
2
;
Tuul N
3
Author Information
1. National Center for Public Health
2. NUM, School of Science, Department of Biology
3. MNUMS, School of Public Health
- Publication Type:Journal Article
- From:
Health Laboratory
2021;14(2):5-12
- CountryMongolia
- Language:Mongolian
-
Abstract:
Background:Soil pollution in Ulaanbaatar has increased in recent years, causing infectious and non-communicable diseases, both benign and malignant.
Therefore, in order to reduce soil pollution, it is necessary to isolate strains resistant to heavy metals and use these microorganisms capable to degrade or accumulate heavy metals for bioremediation purposes.
Methods:Bacterial pure cultures were isolated from soil and water samples and incubated in a Nutrient Broth medium with a lead concentration of 1000 mg/L in a thermostat with a shaker at 37°C. The growth rate was determined at a wavelength of 600 nm using a Spectrophotometer.
Results:In total 69 pure cultures were isolated from the soil samples and 65 pure cultures from them were able to grow at the concentration of lead of 1 mmol/L. 4 pure cultures (UBLF1, UBLF3, UBMF2, and SUBZ4) were able tolerate lead concentrations up to 8 mM. These cultures were determined by semi-automatic VITEK®2 analyzers and identified as Bacillus sp. (83%) and UBMF2 identified as Bacillus thuringiensis (93%) respectively.
Conclusion:The lead content in water samples were found to be acceptable, and isolated pure cultures were not able to withstand the lead concentrations.
Pure culture UBLF3 isolated from the soil capable to reduce lead concentration (500 mg/L) up to 302 mg/L and 230 mg/L in the medium, and pure culture UBMF2 up to 340 mg/L and 279 mg/L respectively
- Full text:HL-2021-14(2)-5-12.pdf
