Effects of Storage Conditions on Complete Blood Cell Count Parameters
- VernacularTitle:Цусны дэлгэрэнгүй шинжилгээний үзүүлэлтэд хадгалах нөхцөл нөлөөлөх нь
- Author:
Batchimeg N
1
;
Oyunkhand L
2
;
Altankhuyag E
2
;
Gantulga D
1
;
Uranbaigali E
1
;
Munkhtulga L
1
Author Information
1. SOM,MNUMS
2. MJH, MNUMS
- Publication Type:Journal Article
- From:
Health Laboratory
2020;11(1):18-23
- CountryMongolia
- Language:Mongolian
-
Abstract:
Introduction:The complete blood count (CBC) is a frequently performed laboratory test today. This study evaluated the effects of temperature and sample storage time on parameters of CBC which could produce misleading results of clinical significance.
Methods:In a cross-sectional study, CBC was checked in 20 randomly selected out-patients and baseline measurements were analyzed using the XN-2000 (Sysmex, Kobe, Japan) fully automated hematology analyzer. CBC was done all samples of storage at room temperature. Values were checked at time intervals of 0, 6, and 24 hr.
Results:Among CBC parameters, white blood cell, red blood cell, hemoglobin, mean cell hemoglobin (MCH), neutrophils and lymphocytes were stable at time up to 6 h. Hematocrit increased between 0 and 24 hours, averaging 41.5% and 45.2%, respectively. MCV, RDW-SD, and RDW-CV increased between 0 and 24 hours. The mean value was statistically significant. There were 85.6fL/ 93.4fL (p<0.001), 40.7fL /48.2fL (p<0.001), 13.1% and 14.2% (p<0.05), respectively.
However, the MCHC was affected by time differences. (p <0.001 at 0 and 24 hours, p <0.001 at 3 and 24 hours). Platelet PDW, MPV, and P-LCR values increased between 0 and 24 h, respectively.
Conclusion:Whole blood samples were stored at room temperature for 24 hours for CBC tests, there were statistically significant differences in the size of red blood cells and platelets.
- Full text:HL-2020-11(1)-18-23.pdf
