Role of negative regulators on the TLR7 ligand/IFN-γ signaling in the endothelial cells

Baasansuren E; Javkhlan B; Baljinnyam T; Khulan U; Batkhishig M; Enkhsaikhan L; Ulziisaikhan J; Khongorzul B; Baigalmaa B; Galindev B; Tsevelmaa N; Sodnomtsogt L; Nyambayar D; Munkhtuvshin N; Munkhbat B; Bilegtsaikhan Ts

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Role of negative regulators on the TLR7 ligand/IFN-γ signaling in the endothelial cells

VernacularTitle:TLR7-ийн лиганд болон IFN-γ-ийн дохио дамжилтын хоорондын харилцан үйлчлэлд сөрөг зохицуулагч молекулын нөлөөг тодорхойлох нь
Author: Baasansuren E ^{1
,

2} ; Javkhlan B ^{1
,

2} ; Baljinnyam T ^{2
,

3} ; Khulan U ² ; Batkhishig M ² ; Enkhsaikhan L ² ; Ulziisaikhan J ² ; Khongorzul B ¹ ; Baigalmaa B ¹ ; Galindev B ¹ ; Tsevelmaa N ¹ ; Sodnomtsogt L ¹ ; Nyambayar D ¹ ; Munkhtuvshin N ³ ; Munkhbat B ¹ ; Bilegtsaikhan Ts ¹
Author Information

1. Core Laboratory, Science Technology Center, MNUMS
2. Department of Microbiology and Immunology, SBS, MNUMS
3. Central Scientific Research Laboratory, Institute of Medical Sciences, Mongolia
Publication Type:Journal Article
From: Health Laboratory 2018;8(1):14-18
CountryMongolia
Language:Mongolian
Abstract: Introduction:Toll like receptors (TLRs) are a class of proteins that key role in the innate immune system. The SOCS1 and SHP2 proteins are negative-feed loop inhibitors of signaling of JAK/STAT and TLRs pathways.
Purpose:To determine negative regulator protein activation which is activated through TLR7 ligand/IFN-γ signal transduction in endothelial cells.
Methods:We used mouse aortic linear endothelial cell (END-D); protein expressio was detected by western blotting
Results:We analyzed a time dependent stimulation effects of negative regulator proteins stimulated by TLR7 ligand/IFN-γ in endothelial cell cultures. Imiquimod of 10 μg/ml treatment of 1 hr was followed by 100 ng/ml IFN-γ stimulation for 1-8hr to analysis of negative regulator SOCS1 and SHP2 protein expression.
In untreated cells, there was low activations of negative regulator SOCS1 and SHP2 proteins. IFN-γ stimulation alone had increased SOCS1 and SHP2 protein expressions, also imiquimod treatment highly elevated SOCS1 and SHP2 expressions. However imiquimod and IFN-γ doubled treatment have decreased activation of negative regulator SOCS1 and SHP2 proteins. These findings suggest SOCS1 and SHP2 proteins are inhibitors in the TLR7 ligand/IFN-γ signaling.
Conclusion:Negative regulators, SOCS1 and SHP2 strongly suppressed activations of TLR7 ligand/IFN-γ signaling
Full text:HL-2018-8(2)-14-18.pdf